Department of Plastic and Reconstructive Surgery, Ninth People's Hospital affiliated to Medical School of Shanghai Jiao Tong University, 639 Zhi Zao Ju Road, Shanghai 200011, PR China.
Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Biomaterials. 2016 Mar;83:169-81. doi: 10.1016/j.biomaterials.2016.01.002. Epub 2016 Jan 4.
Incorporation of bioactive drugs and biofunctionalization of polyester fibrous scaffolds are essential means to improve their bio-functions and histocompatibility for regenerative medicine. However, it is still a challenge to biofunctionalize such drug carriers via traditional biochemical methods while maintaining their properties without changes in drug activity and loading ratio. Here, we demonstrated a facile approach for biofunctionalization of PLGA fibrous scaffolds with various molecules (i.e., PEG polymer, RGD peptide and bFGF growth factor for cell repellent, adhesion and proliferation, respectively) via mussel-Inspired poly(dopamine) (PDA) coating in aqueous solution. By virtue of the mild and efficient nature of this approach, the drug-loaded PLGA fibers could be easily biofunctionalized and showed negligible effects on the scaffold properties, especially drug activity and loading ratio. Further, in vivo study showed that, a ginsenoside-Rg3-loaded fibrous scaffold functionalized with bFGF growth factor could not only promote the early-stage wound healing in rabbit ear wounds (bio-signal from bFGF), but also inhibit later-stage hypertrophic scars formation (release of Rg3 drug). Therefore, the mussel-inspired method for bio-modification provides a facile and effective strategy to combine drug and bio-function in one system, thus facilitating a synergistic effect of drug-therapy and bio-signal when such biomaterial is used for regenerative medicine.
将生物活性药物掺入和聚酯纤维支架的生物功能化是改善其生物功能和组织相容性以用于再生医学的重要手段。然而，通过传统的生化方法对这些药物载体进行生物功能化，同时保持其性质不变，而不改变药物活性和载药量，仍然是一个挑战。在这里，我们展示了一种简便的方法，通过贻贝启发的聚多巴胺（PDA）在水溶液中涂层，可将各种分子（例如 PEG 聚合物、RGD 肽和 bFGF 生长因子，分别用于细胞排斥、粘附和增殖）生物功能化到 PLGA 纤维支架上。由于这种方法温和高效，载药的 PLGA 纤维可以很容易地进行生物功能化，并且对支架性能几乎没有影响，特别是药物活性和载药量。此外，体内研究表明，负载有人参皂苷 Rg3 的纤维支架用 bFGF 生长因子进行功能化不仅可以促进兔耳伤口的早期愈合（来自 bFGF 的生物信号），还可以抑制后期的增生性瘢痕形成（Rg3 药物的释放）。因此，贻贝启发的生物修饰方法为在一个系统中结合药物和生物功能提供了一种简便有效的策略，从而在将此类生物材料用于再生医学时促进药物治疗和生物信号的协同作用。
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