Ivanova Elena, Fayzullin Alexey, Minaev Nikita, Dolganova Irina, Serejnikova Natalia, Gafarova Elvira, Tokarev Mark, Minaeva Ekaterina, Aleksandrova Polina, Reshetov Igor, Timashev Peter, Shekhter Anatoly
Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., Moscow 119991, Russia.
B.V. Petrovsky Russian Research Center of Surgery, 2 Abrikosovskiy Lane, Moscow 119991, Russia.
Polymers (Basel). 2023 Oct 17;15(20):4119. doi: 10.3390/polym15204119.
The formation of a dense fibrous capsule around the foreign body and its contracture is the most common complication of biomaterial implantation. The aim of our research is to find out how the surface of the implant influences the inflammatory and fibrotic reactions in the surrounding tissues. We made three types of implants with a remote surface topography formed of polylactide granules with different diameters: large (100-200 µm), medium (56-100 µm) and small (1-56 µm). We placed these implants in skin pockets in the ears of six chinchilla rabbits. We explanted the implants on the 7th, 14th, 30th and 60th days and performed optical coherence tomography, and histological, immunohistochemical and morphometric studies. We examined 72 samples and compared the composition of immune cell infiltration, vascularization, the thickness of the peri-implant tissues, the severity of fibrotic processes and α-SMA expression in myofibroblasts. We analyzed the scattering coefficient of tissue layers on OCT scans. We found that implants made from large granules induced a milder inflammatory process and slower formation of a connective tissue capsule around the foreign body. Our results prove the importance of assessing the surface texture in order to avoid the formation of capsular contracture after implantation.
异物周围致密纤维囊的形成及其挛缩是生物材料植入最常见的并发症。我们研究的目的是找出植入物表面如何影响周围组织中的炎症和纤维化反应。我们制作了三种具有由不同直径的聚丙交酯颗粒形成的微观表面形貌的植入物：大颗粒（100 - 200 µm）、中颗粒（56 - 100 µm）和小颗粒（1 - 56 µm）。我们将这些植入物置于六只龙猫兔耳朵的皮肤袋中。在第7天、14天、30天和60天取出植入物，并进行光学相干断层扫描以及组织学、免疫组织化学和形态计量学研究。我们检查了72个样本，并比较了免疫细胞浸润的组成、血管化、植入物周围组织的厚度、纤维化过程的严重程度以及肌成纤维细胞中α - SMA的表达。我们分析了光学相干断层扫描图像上组织层的散射系数。我们发现由大颗粒制成的植入物引发的炎症过程较轻，异物周围结缔组织囊的形成较慢。我们的结果证明了评估表面质地对于避免植入后包膜挛缩形成的重要性。
