Liu Ming-dong, Shen Yong-hua
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2015 Nov;35(11):1361-6.
To explore the effect of Tanshinone II A on severe acute pancreatitis (SAP) lung injury (ALI) rats and its possible mechanism.
SD rats were injected with sodium taurocholate to induce SAP group, and then intervened with sodium tanshinone II A sulfonate ( STS group). Simultaneously a sham-operation group (SO group) was set up. There were 24 rats in each group. The survival state and wet-to-dry weight ratio of lung tissues were observed. Activities of myeloperoxidase (MPO) in lung were determined by MPO reagent kit. Pathologic changes of lung tissues were determined by Hofbuaer method. Expression levels of three cytokines, TNF-alpha, IL-1beta, and intercellular cell adhesion molecule-1 (ICAM-1) were detected by ELISA.
The survival state of rats in the SAP group was deteriorated. The wet-to-dry weight ratio, MPO activities, pathologic changes in lung tissues, and expression levels of TNF-alpha, IL-1beta, and ICAM-1 increased significantly more in the SAP group than in the SO group (P < 0.05). Compared with those in the SAP group, the survival state of rats in the STS group was improved; the wet-to-dry weight ratio, MPO activities, pathologic changes in lung tissues, and expression levels of TNF-alpha, IL-1beta, and ICAM-1 obviously decreased in the STS group (P < 0.05).
Tanshinone II A had remarkable effect on SPA LI rats, which might be associated with changing cytokines levels and attenuating infiltration of lung inflammatory cells.
探讨丹参酮ⅡA对重症急性胰腺炎（SAP）肺损伤（ALI）大鼠的影响及其可能机制。
将SD大鼠注射牛磺胆酸钠诱导成SAP组，然后用丹参酮ⅡA磺酸钠干预（STS组）。同时设立假手术组（SO组）。每组24只大鼠。观察大鼠生存状态及肺组织湿干重比。用髓过氧化物酶（MPO）试剂盒测定肺组织中MPO活性。采用Hofbuaer法观察肺组织病理变化。用酶联免疫吸附测定法（ELISA）检测肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和细胞间黏附分子-1（ICAM-1）三种细胞因子的表达水平。
SAP组大鼠生存状态恶化。与SO组相比，SAP组大鼠肺组织湿干重比、MPO活性、病理变化以及TNF-α、IL-1β和ICAM-1的表达水平显著升高（P<0.05）。与SAP组相比，STS组大鼠生存状态改善；STS组大鼠肺组织湿干重比、MPO活性、病理变化以及TNF-α、IL-1β和ICAM-1的表达水平明显降低（P<0.05）。
丹参酮ⅡA对SPA-LI大鼠有显著作用，可能与改变细胞因子水平及减轻肺炎症细胞浸润有关。
