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喉镜:对喉镜叶片微生物负荷的评估。

Laryngoscopes: Evaluation of microbial load of blades.

作者信息

Negri de Sousa Ana Claudia, Vilas Boas Vanessa Aparecida, Levy Carlos Emilio, Pedreira de Freitas Maria Isabel

机构信息

Operating Theater, Women's Hospital Prof. Dr. José Aristodemo Pinotti, CAISM-University of Campinas, Campinas, SP, Brazil.

Infection Control Commission, Women's Hospital Prof. Dr. José Aristodemo Pinotti, CAISM-University of Campinas, Campinas, SP, Brazil.

出版信息

Am J Infect Control. 2016 Mar 1;44(3):294-8. doi: 10.1016/j.ajic.2015.10.014. Epub 2016 Jan 5.

DOI:10.1016/j.ajic.2015.10.014
PMID:26775934
Abstract

BACKGROUND

Laryngoscope blades were analyzed, and the presence of blood, bodily fluids, and microorganisms was verified, indicating their potential as a source of cross contamination during clinical usage. The way in which the blades are cleaned and disinfected in daily practice may place the patient and health care team at risk. The aim of this study was to determine the bacterial and fungal load on this equipment.

METHODS

Descriptive cross-sectional study. A total of 83 laryngoscope blades, ready for use, were analyzed for their bacterial and fungal load at 2 university hospitals.

RESULTS

The microbiologic analysis revealed the presence of microorganisms in 76.2% of cases at institution 1 and 92.7% of cases at institution 2, with microbial loads >10(1) colony forming units in 31.2% and 44.7% of cases, respectively. At both institutions, potentially pathogenic microorganisms were found, including Candida sp, Staphylococcus aureus, Enterococcus faecalis, Streptococcus agalactiae, extended-spectrum β-lactamase-producing Klebsiella pneumoniae, multiresistant Acinetobacter baumannii, Pantoea sp, Enterobacter gergoviae, Escherichia coli, and Proteus mirabilis.

CONCLUSIONS

These results indicate that the use of laryngoscope blades at these 2 institutions present a potential risk. Based on these findings, action needs to be taken so a higher level of safety can be offered to patients and health care professionals who have direct contact with this equipment.

摘要

背景

对喉镜叶片进行了分析,证实存在血液、体液和微生物,这表明它们在临床使用期间有可能成为交叉污染的源头。在日常实践中,喉镜叶片的清洁和消毒方式可能会使患者和医护团队面临风险。本研究的目的是确定该设备上的细菌和真菌负荷。

方法

描述性横断面研究。在2所大学医院对总共83个准备使用的喉镜叶片进行了细菌和真菌负荷分析。

结果

微生物学分析显示,机构1中76.2%的病例以及机构2中92.7%的病例存在微生物,微生物负荷>10(1)菌落形成单位的病例分别占31.2%和44.7%。在这两所机构中,均发现了潜在的致病微生物,包括念珠菌属、金黄色葡萄球菌、粪肠球菌、无乳链球菌、产超广谱β-内酰胺酶的肺炎克雷伯菌、多重耐药的鲍曼不动杆菌、泛菌属、杰氏肠杆菌、大肠埃希菌和奇异变形杆菌。

结论

这些结果表明,在这两所机构中使用喉镜叶片存在潜在风险。基于这些发现,需要采取行动,以便为直接接触该设备的患者和医护人员提供更高水平的安全保障。

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