Lamberti Cristina, Acquadro Elena, Corpillo Davide, Giribaldi Marzia, Decastelli Lucia, Garino Cristiano, Arlorio Marco, Ricciardi Carlo, Cavallarin Laura, Giuffrida Maria Gabriella
ISPA-CNR, Bioindustry Park S. Fumero, Via Ribes 5, 10010 Colleretto Giacosa, Turin, Italy.
Food Chem. 2016 May 15;199:119-27. doi: 10.1016/j.foodchem.2015.11.130. Epub 2015 Nov 30.
A simple validated LC-MS/MS-based method was set up to detect milk contamination in bakery products, taking the effects of food processing into account for the evaluation of allergen recovery and quantification. Incurred cookies were prepared at eight levels of milk contamination and were cooked to expose all milk components, including allergenic proteins, to food processing conditions. Remarkable results were obtained in term of sufficiently low LOD and LOQ (1.3 and 4 mg/kg cookies, respectively). Precision was calculated as intra-day repeatability (RSD in the 5-20% range) and inter-day repeatability (4 days; RSD never exceeded 12%). The extraction recovery values ranged from 20% to 26%. Method applicability was evaluated by analysing commercial cookies labelled either as "milk-free" or "may contain milk". Although the ELISA methodology is considered the gold standard for detecting allergens in foods, this robust LC-MS/MS approach should be a useful confirmatory method for assessing and certifying "milk-free" food products.
建立了一种基于液相色谱-串联质谱（LC-MS/MS）的简单验证方法，用于检测烘焙食品中的牛奶污染，同时考虑食品加工对过敏原回收率和定量评估的影响。制备了八个牛奶污染水平的实际饼干样本，并进行烘焙，以使所有牛奶成分（包括致敏蛋白）都暴露于食品加工条件下。在足够低的检测限（LOD）和定量限（LOQ）（分别为1.3和4mg/kg饼干）方面取得了显著成果。精密度以日内重复性（相对标准偏差（RSD）在5%-20%范围内）和日间重复性（4天；RSD从未超过12%）计算。提取回收率在20%至26%之间。通过分析标记为“无牛奶”或“可能含有牛奶”的市售饼干来评估方法的适用性。尽管酶联免疫吸附测定（ELISA）方法被认为是检测食品中过敏原的金标准，但这种强大的LC-MS/MS方法对于评估和认证“无牛奶”食品应是一种有用的确认方法。
