Yasir Muhammad, Sultana Bushra, Nigam Poonam Singh, Owusu-Apenten Richard
Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan.
Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan; Department of Chemistry, University of California, CA 95616, USA.
Food Chem. 2016 May 15;199:307-13. doi: 10.1016/j.foodchem.2015.11.138. Epub 2015 Nov 30.
Cucurbitaceae are one of most widely used plant species for human food but lesser known members have not been examined for bioactive components. The purpose of this study was to evaluate the antioxidant and genoprotective activities from three cucurbitaceae seeds extracts and to identify phenolic components by LC-ESIMS/MS analysis. From the results, the yield of seeds extract was 20-41% (w/w) and samples had 16-40% total phenols as gallic acid equivalents (GAE). Compared with methanol solvent, using acidified methanol led to increased extraction yield by 1.4 to 10-fold, higher phenolic content (149.5 ± 1.2 to 396.4 ± 1.9 mg GAE/g), higher DPPH radical quenching and enhanced genoprotective activity using the pBR322 plasmid assay. LC-ESI-MS/MS analysis led to identification of 14-17 components, based on authentic standards and comparison with literature reports, as mainly phenolic acids and esters, flavonol glycosides. This may be the first mass spectrometric profiling of polyphenol components from cucurbitaceae seeds.
葫芦科植物是人类食物中使用最广泛的植物种类之一，但鲜为人知的成员尚未对其生物活性成分进行研究。本研究的目的是评估三种葫芦科植物种子提取物的抗氧化和基因保护活性，并通过液相色谱-电喷雾电离串联质谱（LC-ESIMS/MS）分析鉴定酚类成分。结果表明，种子提取物的得率为20-41%（w/w），样品中总酚含量以没食子酸当量（GAE）计为16-40%。与甲醇溶剂相比，使用酸化甲醇可使提取率提高1.4至10倍，酚类含量更高（149.5±1.2至396.4±1.9 mg GAE/g），DPPH自由基淬灭能力更强，并通过pBR322质粒分析增强了基因保护活性。基于标准品并与文献报道进行比较，LC-ESI-MS/MS分析鉴定出了主要为酚酸和酯类、黄酮醇苷类的14-17种成分。这可能是首次对葫芦科植物种子中的多酚成分进行质谱分析。
