Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland.
Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland.
Food Chem. 2016 May 15;199:509-15. doi: 10.1016/j.foodchem.2015.12.041. Epub 2015 Dec 10.
The popularity of Agaricus bisporus results not only from the quality of the flavors, but also from the content of many substances of therapeutic properties. This paper presents a study on RP-HPLC determination of the content of indole compounds released from the lyophilized biomass from in vitro cultures of A. bisporus into artificial digestive juices at 37°C. A. bisporus in vitro cultures were grown on media enriched with zinc salts. The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the greatest number of samples. The content of 5-hydroxy-l-tryptophan in the investigated samples ranged from 86.62 to 531 mg/100g d.w. The amount of l-tryptophan was determined within the range of 1.63-4.68 mg/100g d.w. and for melatonin 0.43-0.64 mg/100g d.w. It was demonstrated for the first time that in vitro cultures of A. bisporus release indole compounds in conditions simulating the human digestive tract.
双孢蘑菇的流行不仅源于其口味的优良，还源于其具有多种治疗特性的物质含量。本文研究了用反相高效液相色谱法（RP-HPLC）测定从体外培养的双孢蘑菇冻干生物量在 37°C 的人工消化液中释放的吲哚化合物的含量。体外培养的双孢蘑菇在富含锌盐的培养基上生长。在大多数样本中发现了 5-羟色氨酸和色氨酸的释放。在所研究的样品中，5-羟色氨酸的含量在 86.62 至 531mg/100g d.w. 之间。色氨酸的含量在 1.63-4.68mg/100g d.w. 范围内，褪黑素的含量为 0.43-0.64mg/100g d.w. 这是首次证明在模拟人体消化道的条件下，体外培养的双孢蘑菇会释放吲哚化合物。
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