Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany, 9 Medyczna Street, 30-688, Kraków, Poland.
Chair of Biochemistry and Biotechnology, Faculty of Biology and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033, Lublin, Poland.
Sci Rep. 2020 Nov 18;10(1):20081. doi: 10.1038/s41598-020-76899-1.
Fomitopsis officinalis is a medicinal mushroom used in traditional European eighteenth and nineteenth century folk medicine. Fruiting bodies of F. officinalis were collected from the natural environment of Świętokrzyskie Province with the consent of the General Director for Environmental Protection in Warsaw. Mycelial cultures were obtained from fragments of F. officinalis fruiting bodies. The taxonomic position of the mushroom mycelium was confirmed using the PCR method. The presence of organic compounds was determined by HPLC-DAD analysis. Bioelements were determined by AF-AAS. The biochemical composition of the tested mushroom material was confirmed with the FTIR method. Antioxidant properties were determined using the DPPH method, and the antiproliferative activity was assessed with the use of the MTT test. The presence of indole compounds (L-tryptophan, 6-methyl-D,L-tryptophan, melatonin, 5-hydroxy-L-tryptophan), phenolic compounds (p-hydroxybenzoic acid, gallic acid, catechin, phenylalanine), and sterols (ergosterol, ergosterol peroxide) as well as trace elements was confirmed in the mycelium and fruiting bodies of F. officinalis. Importantly, a high level of 5-hydroxy-L-tryptophan in in vitro mycelium cultures (517.99 mg/100 g d.w) was recorded for the first time. The tested mushroom extracts also showed antioxidant and antiproliferative effects on the A549 lung cancer cell line, the DU145 prostate cancer cell line, and the A375 melanoma cell line.
药用真菌美味牛肝菌是一种传统的欧洲十八、十九世纪民间药物。美味牛肝菌子实体来源于华沙环境保护总局同意采集的圣十字省的自然环境。通过从美味牛肝菌子实体的碎片中获得的菌丝体培养物，采用 PCR 方法确定了蘑菇菌丝体的分类地位。通过 HPLC-DAD 分析确定了有机化合物的存在。通过 AF-AAS 确定了生物元素。通过 FTIR 方法确定了测试蘑菇材料的生物化学组成。采用 DPPH 法测定抗氧化性能，采用 MTT 试验评估增殖抑制活性。在美味牛肝菌菌丝体和子实体中确认了吲哚化合物（L-色氨酸、6-甲基-D，L-色氨酸、褪黑素、5-羟基-L-色氨酸）、酚类化合物（对羟基苯甲酸、没食子酸、儿茶素、苯丙氨酸）和甾醇（麦角甾醇、麦角甾醇过氧化物）以及微量元素的存在。重要的是，首次在体外菌丝体培养物中记录到 5-羟基-L-色氨酸的高水平（517.99mg/100g 干重）。测试的蘑菇提取物对肺癌 A549 细胞系、前列腺癌 DU145 细胞系和黑色素瘤 A375 细胞系也表现出抗氧化和抗增殖作用。
