Phillips S E, Manfield I, Parsons I, Davidson B E, Rafferty J B, Somers W S, Margarita D, Cohen G N, Saint-Girons I, Stockley P G
Astbury Department of Biophysics, University of Leeds, UK.
Nature. 1989 Oct 26;341(6244):711-5. doi: 10.1038/341711a0.
We present biochemical and genetic data to support the hypothesis that the Escherichia coli met repressor, MetJ, binds to synthetic and natural operator sequences in tandem arrays such that repression depends not only on the affinity of the DNA-protein interaction, but also on protein-protein contacts along the tandem array. This represents a novel form of regulatory switch. Furthermore, there seems to be homology between the organization of the met and trp operators.
我们提供了生化和遗传学数据，以支持以下假说：大肠杆菌甲硫氨酸阻遏物MetJ与串联排列的合成及天然操纵序列结合，使得阻遏作用不仅取决于DNA-蛋白质相互作用的亲和力，还取决于沿串联阵列的蛋白质-蛋白质接触。这代表了一种新型的调控开关。此外，甲硫氨酸和色氨酸操纵子的组织之间似乎存在同源性。
