Butts Christine A, Paturi Gunaranjan, Tavendale Michael H, Hedderley Duncan, Stoklosinski Halina M, Herath Thanuja D, Rosendale Douglas, Roy Nicole C, Monro John A, Ansell Juliet
The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand.
The New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand.
Food Funct. 2016 Apr;7(4):1825-32. doi: 10.1039/c5fo01056j.
The fate of stable-isotope (13)C labelled and non-labelled inulin catabolism by the gut microbiota was assessed in a healthy rat model. Sprague-Dawley male rats were randomly assigned to diets containing either cellulose or inulin, and were fed these diets for 3 days. On day (d) 4, rats allocated to the inulin diet received (13)C-labelled inulin. The rats were then fed the respective non-labelled diets (cellulose or inulin) until sampling (d4, d5, d6, d7, d10 and d11). Post feeding of (13)C-labelled substrate, breath analysis showed that (13)C-inulin cleared from the host within a period of 36 hours. Faecal (13)C demonstrated the clearance of inulin from gut with a (13)C excess reaching maximum at 24 hours (d5) and then declining gradually. There were greater variations in caecal organic acid concentrations from d4 to d6, with higher concentrations of acetic, butyric and propionic acids observed in the rats fed inulin compared to those fed cellulose. Inulin influenced caecal microbial glycosidase activity, increased colon crypt depth, and decreased the faecal output and polysaccharide content compared to the cellulose diet. In summary, the presence of inulin in the diet positively influenced large bowel microbial fermentation.
在健康大鼠模型中评估了肠道微生物群对稳定同位素（13）C标记和未标记菊粉的分解代谢命运。将Sprague-Dawley雄性大鼠随机分配到含有纤维素或菊粉的饮食中，并喂食这些饮食3天。在第4天，分配到菊粉饮食组的大鼠接受（13）C标记的菊粉。然后给大鼠喂食各自的未标记饮食（纤维素或菊粉）直至取样（第4、5、6、7、10和11天）。在喂食（13）C标记的底物后，呼吸分析表明（13）C-菊粉在36小时内从宿主体内清除。粪便中的（13）C表明菊粉从肠道清除，（13）C过量在24小时（第5天）达到最大值，然后逐渐下降。从第4天到第6天，盲肠有机酸浓度的变化更大，与喂食纤维素的大鼠相比，喂食菊粉的大鼠中乙酸、丁酸和丙酸的浓度更高。与纤维素饮食相比，菊粉影响盲肠微生物糖苷酶活性，增加结肠隐窝深度，并减少粪便输出和多糖含量。总之，饮食中菊粉的存在对大肠微生物发酵有积极影响。
