Rho 相关激酶抑制剂通过 ERK 信号通路促进小胶质细胞摄取。
Rho-Associated Kinase Inhibitors Promote Microglial Uptake Via the ERK Signaling Pathway.
机构信息
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Key Laboratory of Neurological Diseases (HUST), Ministry of Education of China, Wuhan, 430030, China.
出版信息
Neurosci Bull. 2016 Feb;32(1):83-91. doi: 10.1007/s12264-016-0013-1. Epub 2016 Jan 18.
Microglia are immunocompetent cells in the central nervous system that take up tissue debris and pathogens. Rho-associated kinase (ROCK) has been identified as an important regulator of uptake, proliferation, secretion, and differentiation in a number of cell types. Although ROCK plays critical roles in the microglial secretion of inflammatory factors, migration, and morphology, its effects on microglial uptake activity have not been well characterized. In the present study, we found that treatment of BV2 microglia and primary microglia with the ROCK inhibitors Y27632 and fasudil increased uptake activity and was associated with morphological changes. Furthermore, western blots showed that this increase in uptake activity was mediated through the extracellular-signal-regulated kinase (ERK) signaling cascade, indicating the importance of ROCK in regulating microglial uptake activity.
小胶质细胞是中枢神经系统中的免疫活性细胞，可吞噬组织碎片和病原体。Rho 相关激酶（ROCK）已被确定为多种细胞类型中摄取、增殖、分泌和分化的重要调节剂。虽然 ROCK 在小胶质细胞炎症因子的分泌、迁移和形态中发挥着关键作用，但它对小胶质细胞摄取活性的影响尚未得到很好的描述。在本研究中，我们发现 ROCK 抑制剂 Y27632 和 fasudil 处理 BV2 小胶质细胞和原代小胶质细胞可增加摄取活性，并伴有形态变化。此外，Western blot 显示，这种摄取活性的增加是通过细胞外信号调节激酶（ERK）信号级联介导的，表明 ROCK 在调节小胶质细胞摄取活性中的重要性。
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