Department of Experimental Laboratory, General Hospital of Shenyang Military Command, Shenyang, China.
Hum Vaccin Immunother. 2012 Sep;8(9):1250-8. doi: 10.4161/hv.20579. Epub 2012 Aug 6.
The aim of present research is to analyze the detailed changes of dendritic cells (DCs) induced by pidotimod(PTD). These impacts on DCs of both bone marrow derived DCs and established DC2.4 cell line were assessed with use of conventional scanning electron microscopy (SEM), flow cytometry (FCM), transmission electron microscopy (TEM), cytochemistry assay FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We demonstrated the ability of PTD to induce DC phynotypic and functional maturation as evidenced by higher expression of key surface molecules such as MHC II, CD80 and CD86. The functional tests proved the downregulation of ACP inside the DCs, occurred when phagocytosis of DCs decreased, with simultaneously antigen presentation increased toward maturation. Finally, PTD also stimulated production of more cytokine IL-12 and less TNF-α. Therefore it is concluded that PTD can markedly exert positive induction to murine DCs.
本研究旨在分析匹多莫德（PTD）诱导树突状细胞（DCs）的详细变化。使用常规扫描电子显微镜（SEM）、流式细胞术（FCM）、透射电子显微镜（TEM）、细胞化学测定 FITC-葡聚糖、生物测定和酶联免疫吸附测定（ELISA）评估 PTD 对骨髓来源 DCs 和已建立的 DC2.4 细胞系的这些影响。我们证明了 PTD 诱导 DC 表型和功能成熟的能力，这表现在关键表面分子如 MHC II、CD80 和 CD86 的更高表达上。功能测试证明，当 DCs 的吞噬作用降低时，ACP 在 DC 内的下调，同时抗原呈递增加，向成熟方向发展。最后，PTD 还刺激产生更多的细胞因子 IL-12 和更少的 TNF-α。因此，可以得出结论，PTD 可以显著地对小鼠 DCs 发挥积极诱导作用。
