van der Lelie D, Bron S, Venema G, Oskam L
Department of Molecular Genetics, University of Groningen, Haren, The Netherlands.
Nucleic Acids Res. 1989 Sep 25;17(18):7283-94. doi: 10.1093/nar/17.18.7283.
Plasmids pMV158 and pTB913, originating from Streptococcus agalactiae and a thermophilic Bacillus respectively, were sequenced to completion. Both contained a BA3-type minus origin of replication and an RSA-site, believed to constitute a site-specific recombination site. These two regions were more than 99% homologous to the corresponding regions of the Staphylococcus aureus plasmid pUB110. Deleting the BA3-type minus origin resulted in the accumulation of a considerable amount of single-stranded DNA, both in L. lactis subsp. lactis and B. subtilis, indicating that this minus origin was functional in both bacterial species. Like pUB110, both plasmids contained an open reading frame encoding a putative plasmid recombination enzyme (Pre protein), which was located downstream of the RSA-site. On the basis of sequence comparisons between pUB110, pMV158, pTB913, pT181, pE194, pNE131 and pT48 two distinct families of RSA-sites and Pre proteins could be distinguished.
分别源自无乳链球菌和嗜热芽孢杆菌的质粒pMV158和pTB913已完成测序。二者均含有一个BA3型负向复制起点和一个RSA位点，据信该位点构成一个位点特异性重组位点。这两个区域与金黄色葡萄球菌质粒pUB110的相应区域有超过99%的同源性。删除BA3型负向复制起点导致在乳酸乳球菌乳亚种和枯草芽孢杆菌中均积累了大量单链DNA，这表明该负向复制起点在这两种细菌中均有功能。与pUB110一样, 这两种质粒均含有一个开放阅读框，其编码一种假定的质粒重组酶（Pre蛋白），该开放阅读框位于RSA位点的下游。基于pUB110、pMV158、pTB913、pT181、pE194、pNE131和pT48之间的序列比较，可以区分出两个不同的RSA位点家族和Pre蛋白家族。
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