Murai M, Miyashita H, Araki H, Seki T, Oshima Y
Department of Fermentation Technology, Faculty of Engineering, Osaka University, Japan.
Mol Gen Genet. 1987 Nov;210(1):92-100. doi: 10.1007/BF00337763.
The structure of a 1.5-kb DNA sequence that is necessary and sufficient for the replication of an 8.2-kb cryptic plasmid, pFTB14, isolated from a strain of Bacillus amyloliquefaciens has been characterized. The 1.5-kb DNA sequence contains an open reading frame, rep, stretching for 1017 bp, a promoter region for rep expression, and a possible replication origin for the plasmid upstream of the promoter. The rep product is trans-active and essential for plasmid replication. The predicted rep protein is a basic protein, as are the RepC protein of pT181, RepB of pUB110 and protein A of pC194 (all these found in staphylococci) and the pi protein of the R6K plasmid of Escherichia coli. The predicted rep protein has highly homologous amino acid sequences with protein A of pC194 and RepB of pUB110 throughout the protein molecule, but not with RepC of pT181, pi of R6K or protein RepH encoded by and initiating the replication of pC194.
已对从解淀粉芽孢杆菌菌株中分离出的8.2 kb隐蔽质粒pFTB14进行复制所必需且足够的1.5 kb DNA序列结构进行了表征。该1.5 kb DNA序列包含一个延伸1017 bp的开放阅读框rep、rep表达的启动子区域以及启动子上游质粒的一个可能复制起点。rep产物具有反式活性，对质粒复制至关重要。预测的rep蛋白是一种碱性蛋白，与pT181的RepC蛋白、pUB110的RepB蛋白和pC194的蛋白A（均在葡萄球菌中发现）以及大肠杆菌R6K质粒的π蛋白一样。预测的rep蛋白在整个蛋白质分子中与pC194的蛋白A和pUB110的RepB蛋白具有高度同源的氨基酸序列，但与pT181的RepC、R6K的π或由pC194编码并启动其复制的RepH蛋白不同源。
