Arnal-Pastor M, Pérez-Garnes M, Monleón Pradas M, Vallés Lluch A
Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, C. de Vera s/n, 46022 Valencia, Spain.
Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, C. de Vera s/n, 46022 Valencia, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, Valencia, Spain.
Colloids Surf B Biointerfaces. 2016 Apr 1;140:412-420. doi: 10.1016/j.colsurfb.2016.01.004. Epub 2016 Jan 6.
Scaffolds based on poly(ethyl acrylate) having interwoven channels were coated with a hyaluronan (HA) hydrogel to be used in tissue engineering applications. Controlled typologies of coatings evolving from isolated aggregates to continuous layers, which eventually clog the channels, were obtained by using hyaluronan solutions of different concentrations. The efficiency of the HA loading was determined using gravimetric and thermogravimetric methods, and the hydrogel loss during the subsequent crosslinking process was quantified, seeming to depend on the mass fraction of hyaluronan initially incorporated to the pores. The effect of the topologically different coatings on the scaffolds, in terms of mechanical properties and swelling at equilibrium under different conditions was evaluated and correlated with the hyaluronan mass fraction. The potential of these hydrogel coatings as vehicle for controlled drug release from the scaffolds was validated using a protein model.
基于具有交织通道的聚丙烯酸乙酯的支架被涂上透明质酸（HA）水凝胶，用于组织工程应用。通过使用不同浓度的透明质酸溶液，获得了从孤立聚集体到连续层的涂层控制类型，这些涂层最终会堵塞通道。使用重量法和热重法测定了HA负载效率，并对随后交联过程中的水凝胶损失进行了量化，其似乎取决于最初掺入孔中的透明质酸的质量分数。评估了拓扑结构不同的涂层对支架在不同条件下的机械性能和平衡溶胀方面的影响，并与透明质酸质量分数相关联。使用蛋白质模型验证了这些水凝胶涂层作为从支架中控制药物释放载体的潜力。
