Korzhikov-Vlakh Viktor, Krylova Maria, Sinitsyna Ekaterina, Ivankova Elena, Averianov Ilia, Tennikova Tatiana B
Institute of Chemistry, St. Petersburg State University, Universitetskii Pr. 26, Peterhoff, 198504 St. Petersburg, Russia.
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Pr. 31, 199004 St. Petersburg, Russia.
Polymers (Basel). 2016 Dec 2;8(12):418. doi: 10.3390/polym8120418.
The modification of bioresorbable polyester surfaces in order to alter their biointeractions presents an important problem in biomedical polymer science. In this study, the covalent modification of the surface of poly(lactic acid)-based (PLA-based) films with poly(acryl amide) and sodium alginate hydrogels was performed to change the non-specific polyester interaction with proteins and cells, as well as to make possible the covalent attachment of low-molecular weight ligands and to control protein release. The effect of such modification on the film surface properties was studied. Parameters such as swelling, water contact angle, surface area, and binding capacity of low-molecular weight substances were evaluated and compared. The comparative study of adsorption of model protein (BSA) on the surface of non-modified and modified films was investigated and the protein release was evaluated. Cell viability on the surface of hydrogel-coated films was also tested. The developed approach could be applied for the modification of PLA-based scaffolds for tissue engineering and will be further studied for molecular-imprinting of biomolecules on the surface of polyester-based materials for control of biointeractions.
为改变生物可吸收聚酯表面的生物相互作用而对其进行改性，是生物医学高分子科学中的一个重要问题。在本研究中，用聚丙烯酰胺和海藻酸钠水凝胶对聚乳酸基（PLA基）薄膜表面进行共价改性，以改变聚酯与蛋白质和细胞的非特异性相互作用，使低分子量配体能够共价连接，并控制蛋白质释放。研究了这种改性对薄膜表面性质的影响。对诸如溶胀、水接触角、表面积和低分子量物质的结合能力等参数进行了评估和比较。研究了模型蛋白（牛血清白蛋白，BSA）在未改性和改性薄膜表面的吸附情况，并对蛋白质释放进行了评估。还测试了水凝胶涂层薄膜表面的细胞活力。所开发的方法可用于组织工程中PLA基支架的改性，并将进一步研究用于在聚酯基材料表面进行生物分子的分子印迹以控制生物相互作用。
