Phan Tung Gia, da Costa Antonio Charlys, Del Valle Mendoza Juana, Bucardo-Rivera Filemon, Nordgren Johan, O'Ryan Miguel, Deng Xutao, Delwart Eric
Blood Systems Research Institute, San Francisco, CA, 94118, USA.
Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, 94118, USA.
Arch Virol. 2016 Apr;161(4):959-66. doi: 10.1007/s00705-016-2756-4. Epub 2016 Jan 19.
Viral metagenomics of feces collected from 58 Peruvian children with unexplained diarrhea revealed several small circular ssDNA genomes. Two genomes related to sequences previously reported in feces from chimpanzees and other mammals and recently named smacoviruses were characterized and then detected by PCR in 1.7 % (1/58) and 19 % (11/58) of diarrheal samples, respectively. Another three genomes from a distinct small circular ssDNA viral group provisionally called pecoviruses encoded Cap and Rep proteins with <35 % identity to those in related genomes reported in human, seal, porcine and dromedary feces. Pecovirus DNA was detected in 15.5 % (9/58), 5.9 % (3/51) and 3 % (3/100) of fecal samples from unexplained diarrhea in Peru, Nicaragua and Chile, respectively. Feces containing these ssDNA genomes also contained known human enteric viral pathogens. The cellular origins of these circular ssDNA viruses, whether human cells, ingested plants, animals or fungal foods, or residents of the gut microbiome, are currently unknown.
对58名患有不明原因腹泻的秘鲁儿童的粪便进行病毒宏基因组学研究，发现了几个小的环状单链DNA基因组。有两个基因组与之前在黑猩猩和其他哺乳动物粪便中报道的序列相关，最近被命名为微小病毒，对其进行了特征分析，然后通过PCR分别在1.7%（1/58）和19%（11/58）的腹泻样本中检测到。另外三个来自一个不同的小环状单链DNA病毒组的基因组，暂称为佩科病毒，其编码的Cap和Rep蛋白与在人类、海豹、猪和单峰骆驼粪便中报道的相关基因组中的蛋白一致性低于35%。在秘鲁、尼加拉瓜和智利不明原因腹泻患者的粪便样本中，分别有15.5%（9/58）、5.9%（3/51）和3%（3/100）检测到佩科病毒DNA。含有这些单链DNA基因组的粪便中还含有已知的人类肠道病毒病原体。这些环状单链DNA病毒的细胞来源，无论是人类细胞、摄入的植物、动物或真菌性食物，还是肠道微生物群的居民，目前尚不清楚。
