Chen Wei, Xie Kuixia, Liu Xinhua, Chen Hong
Dermatological Department, Tianjin Fifth Centre Hospital, Tianjin 300450, P.R. China.
School of Biomedical Engineering, Tianjin Medical University, Tianjin 300070, P.R. China.
Mol Med Rep. 2016 Mar;13(3):2327-37. doi: 10.3892/mmr.2016.4790. Epub 2016 Jan 15.
Psoriasis is a common chronic inflammatory, immune-mediated skin disease with a high incidence worldwide. It is a multifactorial disease and its exact pathogenesis has remained largely elusive. The purpose of the present study was to uncover the key pathways and genes associated with the incidence of psoriasis. Gene expression profiles (dataset no. GSE13355) were downloaded from Gene Expression Omnibus. Differentially expressed genes between skin samples from patients with lesional psoriasis or non‑lesional psoriasis and those of normal healthy controls were identified using Bioconductor version 2.13 based in R. Kyoto Encyclopedia of Genes and genomes (KEGG) pathways significantly enriched in patients with lesional psoriasis were identified using gene set enrichment analysis (GSEA). Key KEGG pathways were then identified using leading-edge analysis of the results of GSEA. Differentially expressed genes involved in the significantly enriched KEGG pathways were considered as key genes. Several KEGG pathways which are known to be associated with lesional psoriasis, including autoimmune thyroid disease signaling, natural killer cell-mediated cytotoxicity signaling, as well as several novel pathways, including FCγR-mediated phagocytosis and neurotrophin signaling pathway, were identified. Several verified and novel genes were also got. The present study revealed key pathways and genes associated with psoriasis, which may serve as important biomarkers for the diagnosis and treatment of psoriasis.
银屑病是一种常见的慢性炎症性、免疫介导的皮肤病，在全球发病率较高。它是一种多因素疾病，其确切发病机制在很大程度上仍不清楚。本研究的目的是揭示与银屑病发病相关的关键途径和基因。基因表达谱（数据集编号GSE13355）从基因表达综合数据库下载。使用基于R的Bioconductor 2.13版本，鉴定了银屑病皮损患者或非皮损患者的皮肤样本与正常健康对照者之间的差异表达基因。使用基因集富集分析（GSEA）鉴定了在银屑病皮损患者中显著富集的京都基因与基因组百科全书（KEGG）途径。然后通过对GSEA结果的前沿分析确定关键KEGG途径。参与显著富集的KEGG途径的差异表达基因被视为关键基因。确定了几条已知与银屑病皮损相关的KEGG途径，包括自身免疫性甲状腺疾病信号传导、自然杀伤细胞介导的细胞毒性信号传导，以及几条新途径，包括FcγR介导的吞噬作用和神经营养因子信号通路。还获得了几个已验证的和新的基因。本研究揭示了与银屑病相关的关键途径和基因，这些可能作为银屑病诊断和治疗的重要生物标志物。
