Şahin Saliha
Deparment of Chemistry, Faculty of Science and Arts, University of Uludag, Bursa 16059, Turkey.
Antioxidants (Basel). 2013 Sep 30;2(4):206-15. doi: 10.3390/antiox2040206.
The popularity of fruit tea is increasing in the world because of its antioxidant properties and attractive taste. The aim of this study was to determine and compare the antioxidant property and phenolic composition of 16 different fruit teas. The antioxidant property and total phenol content of fruit teas depending on the extraction condition (water temperature) were examined using the ABTS (2,2-azinobis[3-ethylbenzothiazoline-6-sulphonic acid]) method and the Folin-Ciocalteu method, respectively. The contents of total flavonoid and total anthocyanin of fruit teas was determined by using the UV/Vis spectrophotometric method. The phenolic composition was determined and quantified by using high performance liquid chromatography and photodiode array detection (HPLC-PDA). The highest total phenol content and antioxidant capacity were determined in pomegranate (I). The highest contents of total flavonoid and total anthocyanin were determined in peach (III) and blackberry (I), respectively. Chlorogenic acid, quercetin, myricetin, rutin, rosmarinic acid and ferulic acid were determined in fruit teas. A water temperature of 100 °C was the most effective to extract the highest contents of total phenols, total flavonoids, total anthocyanins and the highest antioxidant capacity in 16 different fruit teas. The purpose of this study was to determine the effect of water temperature on the extraction and quantify the various phenolic compounds in fruit teas by HPLC method for industrial application in producing the extracts.
由于其抗氧化特性和诱人的口感,水果茶在全球越来越受欢迎。本研究的目的是测定和比较16种不同水果茶的抗氧化特性和酚类成分。分别使用ABTS(2,2-联氮-双-(3-乙基苯并噻唑啉-6-磺酸))法和福林-酚法,研究了不同提取条件(水温)下水果茶的抗氧化特性和总酚含量。采用紫外/可见分光光度法测定水果茶中总黄酮和总花青素的含量。通过高效液相色谱和光电二极管阵列检测(HPLC-PDA)对酚类成分进行测定和定量。在石榴(I)中测定出最高的总酚含量和抗氧化能力。在桃子(III)和黑莓(I)中分别测定出最高的总黄酮和总花青素含量。在水果茶中检测到了绿原酸、槲皮素、杨梅素、芦丁、迷迭香酸和阿魏酸。100℃的水温最有利于提取16种不同水果茶中的总酚、总黄酮、总花青素,且具有最高的抗氧化能力。本研究的目的是确定水温对提取的影响,并通过HPLC法对水果茶中的各种酚类化合物进行定量,以便在提取物生产中进行工业应用。