Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 56212, Korea.
Molecules. 2019 Jan 17;24(2):329. doi: 10.3390/molecules24020329.
Ramat is a perennial flowering plant widely cultivated for use in a tea infusion and as a popular beverage. To identify and evaluate the tea infusion made with a γ-irradiated mutant chrysanthemum cultivar with dark purple petals (cv. ARTI-Dark Chocolate), its phytochemical composition and antioxidant activity were tested and compared with those of the commercially available chrysanthemum cultivar with yellow petals (cv. Gamguk) by HPLC-DAD-ESIMS, as well as DPPH and ABTS radical scavenging assays. The purple chrysanthemum tea contained anthocyanins and linarin, which were not detected in the yellow chrysanthemum tea and the content of chlorogenic acid, acacetin-7--β-glucoside, and luteolin was higher compared with the yellow chrysanthemum tea. In contrast, the yellow chrysanthemum tea had higher luteolin-7--β-glucoside, 3,5-dicaffeoylquinic acid, apigenin-7--β-glucoside, and apigenin contents in comparison with the purple chrysanthemum tea. In addition, the content and antioxidant activity of the two chrysanthemum teas were investigated according to different water temperatures and infusing time. The yellow chrysanthemum tea did not show any significant differences according to infusing time and temperature, while the purple chrysanthemum tea was more influenced by the infusing time than water temperature, showing the highest total compound content in the infusing condition of 100 °C and 4 min. Moreover, the floral scent volatiles of the two chrysanthemum tea sources were analyzed using HS-SPME-GC-MS. In the DPPH radical scavenging assay, the purple chrysanthemum tea broadly showed greater antioxidant activity than did the yellow chrysanthemum tea, corresponding to the high content of anthocyanins known as the powerful antioxidant. Further, both chrysanthemum flower teas exhibited strong ABTS radical scavenging effects ranging from 76% to 61% under all infusing conditions. Therefore, the purple chrysanthemum cultivar, ARTI-Dark Chocolate, is worthy of breeding as a new tea cultivar.
紫菀是一种多年生开花植物，广泛种植于茶饮和受欢迎的饮料中。为了鉴定和评估用γ射线辐照的深紫色花瓣菊花品种（ARTI-Dark Chocolate）制成的茶浸提液，通过 HPLC-DAD-ESIMS 以及 DPPH 和 ABTS 自由基清除测定法，对其与市售黄色花瓣菊花品种（Gamguk）的浸提液的植物化学成分和抗氧化活性进行了测试和比较。紫菀茶中含有飞燕草色素和木犀草素，而在黄色菊花茶中未检测到这两种物质，且绿原酸、芹菜素-7--β-葡萄糖苷和木樨草素的含量高于黄色菊花茶。相反，与紫菀茶相比，黄色菊花茶中含有更高的木樨草素-7--β-葡萄糖苷、3,5-二咖啡酰奎宁酸、芹菜素-7--β-葡萄糖苷和芹菜素。此外，还根据不同的水温与冲泡时间对两种菊花茶的含量和抗氧化活性进行了研究。黄色菊花茶的冲泡时间和温度对其含量无明显影响，而紫菀茶则受冲泡时间的影响大于水温，在 100°C 和 4 分钟的冲泡条件下，其总化合物含量最高。此外，采用 HS-SPME-GC-MS 对两种菊花茶来源的花香挥发性成分进行了分析。在 DPPH 自由基清除测定中，紫菀茶的抗氧化活性普遍高于黄色菊花茶，这与作为强大抗氧化剂的飞燕草色素的高含量相对应。此外，两种菊花花茶在所有冲泡条件下，ABTS 自由基清除率均达到 76%至 61%。因此，ARTI-Dark Chocolate 深紫色菊花品种是值得培育的新型茶品种。
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