Tavoosi Zahra, Moradi-Sardareh Hemen, Saidijam Massoud, Yadegarazari Reza, Borzuei Shiva, Soltanian Alireza, Goodarzi Mohammad Taghi
Department of Clinical Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan 65178 38736, Iran.
Department of Biochemistry, Medical School, Tehran University of Medical Sciences, Tehran 14176 13151, Iran.
Cholesterol. 2015;2015:682904. doi: 10.1155/2015/682904. Epub 2015 Dec 15.
ABCA1 and ABCG1 genes encode the cholesterol transporter proteins that play a key role in cholesterol and phospholipids homeostasis. This study was aimed at evaluating and comparing ABCA1 and ABCG1 genes expression in metabolic syndrome patients and healthy individuals. This case-control study was performed on 36 patients with metabolic syndrome and the same number of healthy individuals in Hamadan (west of Iran) during 2013-2014. Total RNA was extracted from mononuclear cells and purified using RNeasy Mini Kit column. The expression of ABCA1 and ABCG1 genes was performed by qRT-PCR. Lipid profile and fasting blood glucose were measured using colorimetric procedures. ABCG1 expression in metabolic syndrome patients was significantly lower (about 75%) compared to that of control group, while for ABCA1 expression, there was no significant difference between the two studied groups. Comparison of other parameters such as HDL-C, FBS, BMI, waist circumference, and systolic and diastolic blood pressure between metabolic syndrome patients and healthy individuals showed significant differences (P < 0.05). Decrease in ABCG1 expression in metabolic syndrome patients compared to healthy individuals suggests that hyperglycemia, related metabolites, and hyperlipidemia over the transporter capacity resulted in decreased expression of ABCG1. Absence of a significant change in ABCA1 gene expression between two groups can indicate a different regulation mechanism for ABCA1 expression.
ABCA1和ABCG1基因编码在胆固醇和磷脂稳态中起关键作用的胆固醇转运蛋白。本研究旨在评估和比较代谢综合征患者与健康个体中ABCA1和ABCG1基因的表达。这项病例对照研究于2013年至2014年期间在伊朗西部哈马丹对36例代谢综合征患者和相同数量的健康个体进行。从单核细胞中提取总RNA，并使用RNeasy Mini试剂盒柱进行纯化。通过qRT-PCR检测ABCA1和ABCG1基因的表达。使用比色法测量血脂谱和空腹血糖。与对照组相比，代谢综合征患者中ABCG1的表达显著降低（约75%），而对于ABCA1的表达，两个研究组之间没有显著差异。代谢综合征患者与健康个体之间其他参数如高密度脂蛋白胆固醇、空腹血糖、体重指数、腰围以及收缩压和舒张压的比较显示出显著差异（P < 0.05）。与健康个体相比，代谢综合征患者中ABCG1表达的降低表明高血糖、相关代谢产物和高脂血症超过转运能力导致ABCG1表达降低。两组之间ABCA1基因表达没有显著变化可能表明ABCA1表达存在不同的调节机制。
