Klimenko Vladimir V, Knyazev Nickolay A, Moiseenko Fedor V, Rusanov Anatoliy A, Bogdanov Alexey A, Dubina Michael V
St Petersburg Academic University, Khlopina str. 8/3, St. Petersburg 194021, Russia.
St Petersburg Academic University, Khlopina str. 8/3, St. Petersburg 194021, Russia; Institute of Cytology, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.
Photodiagnosis Photodyn Ther. 2016 Mar;13:101-107. doi: 10.1016/j.pdpdt.2016.01.003. Epub 2016 Jan 11.
One of the factors limiting photodynamic therapy (PDT) is hypoxia in tumor cells during photodynamic action. PDT with pulse mode irradiation and appropriate irradiation parameters could be more effective in the singlet oxygen generation and tissue re-oxygenation than continuous wave (CW) mode. We theoretically demonstrate differences between the cumulative singlet oxygen concentration in PDT using pulse mode and CW mode of laser irradiation. In vitro experimental results show that photodynamic treatment with pulse mode irradiation has similar cytotoxicity to CW mode and induces mainly cell apoptosis, whereas CW mode induces necrotic cell death. We assume that the cumulative singlet oxygen concentration and the temporal distribution of singlet oxygen are important in photodynamic cytotoxicity and apoptosis initiation. We expect our research may improve irradiation protocols and photodynamic therapy efficiency.
限制光动力疗法（PDT）的因素之一是光动力作用期间肿瘤细胞中的缺氧状态。采用脉冲模式照射及适当照射参数的光动力疗法在单线态氧生成和组织再氧合方面可能比连续波（CW）模式更有效。我们从理论上证明了在使用脉冲模式和连续波模式激光照射的光动力疗法中累积单线态氧浓度的差异。体外实验结果表明，脉冲模式照射的光动力治疗与连续波模式具有相似的细胞毒性，且主要诱导细胞凋亡，而连续波模式则诱导坏死性细胞死亡。我们认为累积单线态氧浓度和单线态氧的时间分布在光动力细胞毒性和凋亡起始中很重要。我们期望我们的研究可能会改善照射方案并提高光动力疗法的效率。
