Sun Dan, Lu Yao, Zhang Su-Juan, Wang Kai-Ge, Sun Zhe
National Center for International Research of Photoelectric Technology and Nano-functional Materials, State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, Institute of Photonics and Photo-Technology Northwest University, Xi'an, 710069, China.
Gen Physiol Biophys. 2017 Oct;36(4):423-430. doi: 10.4149/gpb_2017004. Epub 2017 Aug 24.
At the present time, many cancer patients combine some forms of complementary and alternative medicine therapies with their conventional therapies. The most common choice of these therapies is the use of antioxidants. Formononetin is presented in different foods. It has a variety of biological activities including antioxidant and anti-cancer properties. On account of its antioxidant activity, formononetin might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species (ROS) production was stimulated leading to irreversible tumor cell injury. In this study, the influence of formononetin on K562 cells in PDT was demonstrated. The results showed that formononetin supplementation alone did not affect the lipid peroxidation, DNA damage and apoptosis in K562 cells. It increases the lipid peroxidation, DNA damage and apoptosis in K562 cells induced by PDT. The singlet oxygen quencher sodium azide suppresses the apoptosis induced by PDT with formononetin. In conclusion, formononetin consumption during PDT increases the effectiveness of cancer therapy on malignant cells. The effect of antioxidants on PDT maybe was determined by its sensitization ability to singlet oxygen.
目前，许多癌症患者将某些形式的补充和替代医学疗法与传统疗法相结合。这些疗法最常见的选择是使用抗氧化剂。刺芒柄花素存在于不同的食物中。它具有多种生物活性，包括抗氧化和抗癌特性。由于其抗氧化活性，刺芒柄花素可能在光动力疗法（PDT）中保护癌细胞免受自由基损伤，在此过程中活性氧（ROS）的产生被刺激，导致肿瘤细胞不可逆转的损伤。在本研究中，证明了刺芒柄花素对PDT中K562细胞的影响。结果表明，单独补充刺芒柄花素不会影响K562细胞中的脂质过氧化、DNA损伤和凋亡。它增加了PDT诱导的K562细胞中的脂质过氧化、DNA损伤和凋亡。单线态氧淬灭剂叠氮化钠抑制了刺芒柄花素诱导的PDT凋亡。总之，在PDT期间食用刺芒柄花素可提高癌症治疗对恶性细胞的有效性。抗氧化剂对PDT的作用可能取决于其对单线态氧的敏化能力。
