Slivinska Kateryna, Víchová Bronislava, Werszko Joanna, Szewczyk Tomasz, Wróblewski Zbigniew, Peťko Branislav, Ragač Ondrej, Demeshkant Vitaliy, Karbowiak Grzegorz
I.I. Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine, vul. Bogdana Khmelnitskogo 15, 01601 Kyiv, Ukraine.
Institute of Parasitology of Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
Vet Parasitol. 2016 Jan 15;215:35-7. doi: 10.1016/j.vetpar.2015.10.025. Epub 2015 Oct 29.
A survey was undertaken to assess the prevalence of Theileria equi and Anaplasma phagocytophilum in some regions of Ukraine, Poland and Slovakia. Using a specific PCR assays, blood samples from 215 horses were tested. The prevalence of T. equi and A. phagocytophilum infection was 13.95% and 1.4%, respectively. BLAST analysis showed the isolates closest to the T. equi 18S rRNA and A. phagocytophilum msp4 gene sequences in GenBank with a similarity of ≥99%. No significant association was found between the T. equi PCR positivity and the age or sex of the horses. There was a significant association between the origin of horses and T. equi-PCR positivity. No significant association was found between the A. phagocytophilum-PCR positivity and the age, sex or origin.
开展了一项调查，以评估泰勒焦虫（Theileria equi）和嗜吞噬细胞无形体（Anaplasma phagocytophilum）在乌克兰、波兰和斯洛伐克一些地区的流行情况。使用特异性PCR检测方法，对215匹马的血液样本进行了检测。泰勒焦虫和嗜吞噬细胞无形体感染的流行率分别为13.95%和1.4%。BLAST分析显示，分离株与GenBank中泰勒焦虫18S rRNA和嗜吞噬细胞无形体msp4基因序列最接近，相似度≥99%。未发现泰勒焦虫PCR阳性与马的年龄或性别之间存在显著关联。马的来源与泰勒焦虫PCR阳性之间存在显著关联。未发现嗜吞噬细胞无形体PCR阳性与年龄、性别或来源之间存在显著关联。
