Sanches Gustavo S, Évora Patrícia M, Mangold Atílio J, Jittapalapong Sattaporn, Rodriguez-Mallon Alina, Guzmán Pedro E E, Bechara Gervásio H, Camargo-Mathias Maria I
Instituto de Biociências, Departamento de Biologia, Unesp Campus Rio Claro, Av. 24 A, no. 1515, 13506-900 Rio Claro, SP, Brazil; Laboratório de Imunopatologia, Departamento de Patologia Veterinária, Unesp Campus Jaboticabal, Via de Acesso Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil.
Laboratório de Imunopatologia, Departamento de Patologia Veterinária, Unesp Campus Jaboticabal, Via de Acesso Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil.
Vet Parasitol. 2016 Jan 15;215:78-87. doi: 10.1016/j.vetpar.2015.11.007. Epub 2015 Dec 1.
In this study, different geographical populations of Rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. Phylogenetic trees were constructed using 12S and 16S rDNA sequences and showed two distinct clades: one composed of ticks from Brazil (Jaboticabal, SP), Cuba (Havana) Thailand (Bangkok) and the so-called "tropical strain" ticks. The second clade was composed of ticks from Spain (Zaragoza), Argentina (Rafaela, Santa Fe) and the so-called "temperate strain" ticks. Morphometric analysis showed good separation between females of the two clades and within the temperate clade. Males also exhibited separation between the two clades, but with some overlap. Multiple biological parameters revealed differences between the two clades, especially the weight of the engorged female. These results confirm the existence of at least two species under the name "R. sanguineus".
在本研究中，通过分子、生物学和形态测量方法对不同地理种群的广义血红扇头蜱进行了比较。利用12S和16S rDNA序列构建了系统发育树，结果显示出两个不同的进化枝：一个进化枝由来自巴西（雅博蒂卡巴尔，圣保罗州）、古巴（哈瓦那）、泰国（曼谷）的蜱以及所谓的“热带株”蜱组成。第二个进化枝由来自西班牙（萨拉戈萨）、阿根廷（拉斐拉，圣菲省）的蜱以及所谓的“温带株”蜱组成。形态测量分析表明，两个进化枝的雌性蜱之间以及温带进化枝内部都有良好的区分。雄性蜱在两个进化枝之间也表现出区分，但存在一些重叠。多个生物学参数揭示了两个进化枝之间的差异，尤其是饱血雌蜱的体重。这些结果证实了在“血红扇头蜱”这一名称下至少存在两个物种。
