Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Lisboa, Portugal.
Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Front Cell Infect Microbiol. 2021 Jan 29;10:611113. doi: 10.3389/fcimb.2020.611113. eCollection 2020.
The tick vector is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the "temperate" and "tropical" lineages of (s.l.) is the vector competence to , suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the tropical and temperate lineages and compares their proteomic profile during infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control.
蜱虫媒介被确立为一个具有高度兽医医学意义的密切相关物种复合体，其中不同的遗传、形态和生物学特征的存在导致了分类群内不同谱系的识别。在（s.l.）的“温带”和“热带”谱系之间，最显著的差异之一是对 的媒介能力，这表明这些蜱虫对病原体感染的耐受和反应不同。本研究针对 热带和温带谱系的 SG 和 MG 蛋白质组，并比较了它们在 感染期间的蛋白质组谱。从两个谱系中获得的若虫批次被允许在未感染和实验感染的狗身上吸血，然后蜕皮，成年蜱虫被解剖，分离唾液腺和中肠组织。在进行蛋白质组分析之前，对样本进行了 存在情况的筛查。比较了每个谱系中受感染和未受感染组织中鉴定出的蛋白质的表达，并使用基因本体论对蛋白质进行分类。结果突出了这些蛋白质组谱中的重要差异，这些差异增加了以前报道的遗传、生物学、行为和形态学差异，进一步加强了存在两个不同物种的假说。比较受感染和未受感染的组织，结果表明，虽然在中肠组织中，对 感染的反应在唾液腺中相似，但两个谱系显示出不同的蛋白质表达模式。聚焦于仅在感染条件下发现的蛋白质，数据表明，在蜱虫吸血期间产生的水泥锥体可能与病原体感染有关。本研究为有关有争议的 系统地位的辩论提供了有用的信息，为与两个谱系之间发生的不同媒介能力相关的讨论提供了信息，并确定了有效控制蜱虫和蜱传疾病的潜在目标。
