Burra Gunasekhar, Thakur Ashwani Kumar
Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016, UP, India.
Data Brief. 2015 Nov 14;5:1066-71. doi: 10.1016/j.dib.2015.11.007. eCollection 2015 Dec.
The data provided in this article are related to the research article entitled "Unaided trifluoroacetic acid pretreatment solubilizes polyglutamine (polyGln) peptides and retains their biophysical properties of aggregation" by Burra and Thakur (in press) [1]. This research article reports data from size exclusion chromatography (SEC), reversed phase-high performance liquid chromatography (RP-HPLC) and mass spectrometry (MS) assays. This data show that trifluoroacetic acid (TFA) has the ability to convert insoluble polyGln peptides to soluble monomers. The data also clarify the possibility of trifluoroacetylation modification caused due to TFA. We hope the data presented here will enhance the understanding of polyGln disaggregation and solubilization. For more insightful and useful discussions, see the research article published in Analytical Biochemistry: Methods in the Biological Sciences (Burra and Thakur, in press [1]).
本文提供的数据与Burra和Thakur即将发表的题为《未辅助的三氟乙酸预处理可溶解聚谷氨酰胺（polyGln）肽并保留其聚集的生物物理特性》的研究文章相关[1]。该研究文章报告了尺寸排阻色谱（SEC）、反相高效液相色谱（RP-HPLC）和质谱（MS）分析的数据。这些数据表明，三氟乙酸（TFA）有能力将不溶性聚谷氨酰胺肽转化为可溶性单体。数据还阐明了由TFA引起的三氟乙酰化修饰的可能性。我们希望这里呈现的数据能增进对聚谷氨酰胺解聚和溶解的理解。如需更有见地和有用的讨论，请参阅发表在《分析生物化学：生物科学方法》上的研究文章（Burra和Thakur，即将发表[1]）。
