香菇菌柄壳聚糖的抗菌和抗肿瘤活性与商业蟹壳壳聚糖的比较。
Antimicrobial and antitumor activities of chitosan from shiitake stipes, compared to commercial chitosan from crab shells.
机构信息
Department of Food Science and Biotechnology, National Chung Hsing University (NCHU), Taichung, Taiwan, ROC; NCHU/University of California at Davis, Plant and Food Biotechnology Center, NCHU, Taichung, Taiwan, ROC; Agricultural Biotechnology Center, NCHU, Taiwan, ROC.
Department of Applied Life Science and Health, Chia Nan University of Pharmacy and Science, Jenteh 71710, Tainan, Taiwan, ROC.
出版信息
Carbohydr Polym. 2016 Mar 15;138:259-64. doi: 10.1016/j.carbpol.2015.11.061. Epub 2015 Nov 26.
Chitosan was prepared by alkaline N-deacetylation of chitin obtained from shiitake stipes and crab shells and its antimicrobial and antitumor activities were studied. Chitosan from shiitake stipes and crab shells exhibited excellent antimicrobial activities against eight species of Gram positive and negative pathogenic bacteria with inhibition zones of 11.4-26.8mm at 0.5mg/ml. Among chitosan samples, shiitake chitosan C120 was the most effective with inhibition zones of 16.4-26.8mm at 0.5mg/ml. In addition, shiitake and crab chitosan showed a moderate anti-proliferative effect on IMR 32 and Hep G2 cells. At 5mg/ml, the viability of IMR 32 cells incubated with chitosan was 68.8-85.0% whereas that of Hep G2 cells with chitosan was 60.4-82.9%. Overall, shiitake chitosan showed slightly better antimicrobial and antitumor activities than crab chitosan. Based on the results obtained, shiitake and crab chitosan were strong antimicrobial agents and moderate antitumor agents.
壳聚糖是通过碱性 N-脱乙酰化从香菇菌柄和蟹壳中得到的甲壳素制备的，并研究了其抗菌和抗肿瘤活性。香菇菌柄和蟹壳来源的壳聚糖对 8 种革兰氏阳性和阴性病原菌具有优异的抗菌活性，在 0.5mg/ml 时抑菌圈为 11.4-26.8mm。在壳聚糖样品中，香菇壳聚糖 C120 的效果最为显著，在 0.5mg/ml 时抑菌圈为 16.4-26.8mm。此外，香菇和蟹壳聚糖对 IMR 32 和 Hep G2 细胞表现出适度的抗增殖作用。在 5mg/ml 时，用壳聚糖孵育的 IMR 32 细胞的存活率为 68.8-85.0%，而用壳聚糖孵育的 Hep G2 细胞的存活率为 60.4-82.9%。总体而言，香菇壳聚糖表现出略优于蟹壳聚糖的抗菌和抗肿瘤活性。基于所获得的结果，香菇和蟹壳聚糖是强抗菌剂和适度的抗肿瘤剂。
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