Unité Enzymes et Bioconversion, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, 3038 Sfax, Tunisia.
Unité Enzymes et Bioconversion, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, 3038 Sfax, Tunisia; Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Equipe ProBioGEM, Université Lille 1, France.
Int J Biol Macromol. 2016 Jun;87:163-71. doi: 10.1016/j.ijbiomac.2016.02.057. Epub 2016 Feb 24.
Chitin was recovered through enzymatic deproteinization of the Norway lobster (Nephrops norvegicus) processing by-products. The obtained chitin was characterized and converted into chitosan by N-deacetylation, the acid-soluble form of chitin. Chitosan samples were then characterized by Fourier transform infrared spectroscopy (FTIR) and 13 Cross polarization magic angle spinning nuclear magnetic resonance (CP/MAS)-NMR spectroscopy. The antimicrobial activity and anti-proliferative capacity of chitosan were evaluated. Antimicrobial activity assays indicated that prepared chitosan exhibited marked inhibitory activity against the bacterial and fungal strains tested. Further, cytotoxic effects of chitosan samples on human colon carcinoma cells HCT116 was evaluated using the MTT assay. Chitosan showed the antiproliferative capacity against the colon-cancer-cell HCT116 in a dose dependent manner with IC50 of 4.6mg/ml. Indeed, HCT116 cell proliferation was significantly inhibited (p<0.05) between 13.5 and 67.5% at 0.5-6mg/mL of chitosan after 24h of cell treatment. The chitosan showed high antitumor activity which seemed to be dependent on its characteristics such as acetylation degree.
甲壳素是通过酶解挪威龙虾（Nephrops norvegicus）加工副产物中的蛋白质来回收的。得到的甲壳素经过 N-去乙酰化转化为壳聚糖，这是甲壳素的酸溶性形式。然后通过傅里叶变换红外光谱（FTIR）和 13 交叉极化魔角旋转核磁共振（CP/MAS）-NMR 光谱对壳聚糖样品进行了表征。评估了壳聚糖的抗菌活性和抗增殖能力。抗菌活性测定表明，所制备的壳聚糖对测试的细菌和真菌菌株表现出明显的抑制活性。此外，通过 MTT 测定法评估了壳聚糖样品对人结肠癌细胞 HCT116 的细胞毒性作用。壳聚糖对结肠癌细胞 HCT116 的增殖具有浓度依赖性抑制作用，IC50 为 4.6mg/ml。在用壳聚糖处理细胞 24 小时后，在 0.5-6mg/ml 的浓度范围内，HCT116 细胞的增殖分别显著抑制了 13.5%至 67.5%（p<0.05）。壳聚糖具有高抗肿瘤活性，这似乎与其乙酰化程度等特性有关。
