Lin Jiaofen, Zeng Jian, Shi Guozong, Zhuo Zesheng, Guan Yanyun, Li Zhipeng, Ni Hui, Fei Peng
School of Marine Biology, Xiamen Ocean Vocational College, Applied Technology Engineering Centre of Fujian Provincial Higher Education for Marine Resource Protection and Ecological Governance, Xiamen Key Laboratory of Intelligent Fishery, Xiamen 361100, China.
Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Fujian, Xiamen, 361021, China.
Food Chem X. 2025 Mar 29;27:102425. doi: 10.1016/j.fochx.2025.102425. eCollection 2025 Apr.
This study investigates the laccase-catalyzed grafting of gallic acid (GA) onto shiitake mushroom chitosan to enhance its emulsifying properties and improve β-carotene delivery. Structural characterization using FTIR, XPS, and H NMR revealed that laccase catalysis promoted the formation of amide bonds, disrupted the crystalline structure of chitosan, and enhanced both its hydration and interfacial activity. The modified chitosan emulsions exhibited significantly improved emulsification capacity and stability, with GA-grafted chitosan achieving emulsification activity of 3.34 L/g·cm and stability of 97.6 %. The β-carotene encapsulation efficiency increased to 82.1 %, with enhanced resistance to UV light and H₂O₂-induced degradation. In vitro digestion experiments demonstrated that the modified chitosan emulsion improved β-carotene bioaccessibility (75.8 %) and cellular uptake (55.3 %), significantly improving delivery efficiency. This study provides a novel approach for the development of functional emulsion carriers and lays the foundation for their application in food and drug delivery systems.
本研究考察了漆酶催化没食子酸（GA）接枝到香菇壳聚糖上，以增强其乳化性能并改善β-胡萝卜素的递送。利用傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和核磁共振氢谱（¹H NMR）进行的结构表征表明，漆酶催化促进了酰胺键的形成，破坏了壳聚糖的晶体结构，并增强了其水合作用和界面活性。改性壳聚糖乳液的乳化能力和稳定性显著提高，GA接枝壳聚糖的乳化活性达到3.34 L/g·cm，稳定性为97.6%。β-胡萝卜素的包封率提高到82.1%，对紫外线和过氧化氢诱导的降解具有更强的抗性。体外消化实验表明，改性壳聚糖乳液提高了β-胡萝卜素的生物可及性（75.8%）和细胞摄取率（55.3%），显著提高了递送效率。本研究为功能性乳液载体的开发提供了一种新方法，并为其在食品和药物递送系统中的应用奠定了基础。
