Zan Xingjie, Sitasuwan Pongkwan, Feng Sheng, Wang Qian
Institute of Biomaterials and Engineering, Wenzhou Medical University , Chashan University Town, Wenzhou, Zhejiang Province 325035, P. R. China.
Wenzhou Institute of Biomaterials and Engineering , 16 Xinsan Rd Hi-tech Industry Park, Wenzhou, Zhejiang Province 325011, P. R. China.
Langmuir. 2016 Feb 23;32(7):1808-17. doi: 10.1021/acs.langmuir.5b04245. Epub 2016 Feb 8.
Because of its outstanding osteo-conductive property, a calcium phosphate (CaP) coating has been used as an implant coating for bone tissue engineering. Nevertheless, the issues, such as harsh fabrication conditions, long-term stability and biocompatibility, and the requirement for expensive instruments, still exist in current coating techniques. To address these issues, the CaP coatings doped with collagen (CaP-Col) were in situ generated on polyelectrolyte multilayers (PEMs) by incubating PEMs in a mixture of the collagen, phosphate, and calcium ions. The resulting coatings have controllable physical properties (chemical composition, crystallinity, and roughness) and good stability before and after incubation with cell culture medium. We also found that both the cellular viability and osteogenesis of mesenchymal stem cells (MSCs) were closely related to the roughness of PEMs/CaP-Col, one of the easily ignored physical factors in current coating designs but very critical. The existed roughness window (between 18 ± 1.2 and 187 ± 7.3 nm) suitable for MSC proliferation on PEMs/CaP-Col coating and the optimal roughness (∼98 ± 3.5 nm) for MSC osteogenesis further demonstrated that the roughness was a critical factor for bone formation. Therefore, we envision that our exploration of the effects of surface roughness on MSC behaviors would provide better guidance for the future design of material coating and eventual medical success.
由于具有出色的骨传导性能，磷酸钙（CaP）涂层已被用作骨组织工程的植入物涂层。然而，当前的涂层技术仍然存在诸如苛刻的制造条件、长期稳定性和生物相容性以及对昂贵仪器的需求等问题。为了解决这些问题，通过将聚电解质多层膜（PEMs）在胶原蛋白、磷酸盐和钙离子的混合物中孵育，原位生成了掺杂胶原蛋白的CaP涂层（CaP-Col）。所得涂层具有可控的物理性质（化学成分、结晶度和粗糙度），并且在与细胞培养基孵育前后具有良好的稳定性。我们还发现，间充质干细胞（MSCs）的细胞活力和成骨作用都与PEMs/CaP-Col的粗糙度密切相关，粗糙度是当前涂层设计中一个容易被忽视但非常关键的物理因素。适合MSCs在PEMs/CaP-Col涂层上增殖的粗糙度窗口（在18±1.2和187±7.3nm之间）以及MSCs成骨的最佳粗糙度（约98±3.5nm）进一步证明了粗糙度是骨形成的关键因素。因此，我们设想，我们对表面粗糙度对MSCs行为影响的探索将为未来材料涂层的设计和最终的医学成功提供更好的指导。
