Wattanaanek Nattanan, Suttapreyasri Srisurang, Samruajbenjakun Bancha
Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand.
J Funct Biomater. 2022 Apr 25;13(2):47. doi: 10.3390/jfb13020047.
Congenital abnormalities, trauma, and disease result in significant demands for bone replacement in the craniofacial region and across the body. Tetra-compositions of organic and inorganic scaffolds could provide advantages for bone regeneration. This research aimed to fabricate and characterize amorphous calcium phosphate (ACP)/calcium sulfate hemihydrate (CSH) with alginate/cellulose composite scaffolds using 3D printing. Alginate/cellulose gels were incorporated with 0%, 13%, 15%, 18%, 20%, and 23% ACP/CSH using the one-pot process to improve morphological, physiochemical, mechanical, and biological properties. SEM displayed multi-staggered filament layers with mean pore sizes from 298 to 377 μm. A profilometer revealed mean surface roughness values from 43 to 62 nm that were not statistically different. A universal test machine displayed the highest compressive strength and modulus with a statistical significance in the 20% CP/CS group. FTIR spectroscopy showed peaks in carbonate, phosphate, and sulfate groups that increased as more ACP/CSH was added. Zero percent of ACP/CSH showed the highest swelling and lowest remaining weight after degradation. The 23% ACP/CSH groups cracked after 60 days. In vitro biocompatibility testing used the mouse osteoblast-like cell line MC3T3-E1. The 18% and 20% ACP/CSH groups showed the highest cell proliferation on days five and seven. The 20% ACP/CSH was most suitable for bone cell regeneration.
先天性异常、创伤和疾病导致颅面区域及全身对骨替代物有巨大需求。有机和无机支架的四元组合物可为骨再生提供优势。本研究旨在使用3D打印制造并表征含藻酸盐/纤维素复合支架的无定形磷酸钙（ACP）/半水硫酸钙（CSH）。采用一锅法将藻酸盐/纤维素凝胶与0%、13%、15%、18%、20%和23%的ACP/CSH混合，以改善其形态、物理化学、力学和生物学性能。扫描电子显微镜显示出多交错的丝状层，平均孔径为298至377μm。轮廓仪显示平均表面粗糙度值为43至62nm，无统计学差异。万能试验机显示20% CP/CS组的抗压强度和模量最高，具有统计学意义。傅里叶变换红外光谱显示碳酸盐、磷酸盐和硫酸盐基团的峰随着更多ACP/CSH的添加而增加。0%的ACP/CSH在降解后显示出最高的溶胀度和最低的剩余重量。23% ACP/CSH组在60天后出现破裂。体外生物相容性测试使用小鼠成骨样细胞系MC3T3-E1。18%和20% ACP/CSH组在第5天和第7天显示出最高的细胞增殖。20% ACP/CSH最适合骨细胞再生。
