Lindqvist Julia, Wistbacka Num, Eriksson John E
Cell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland; Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
Cell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland; Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
Methods Enzymol. 2016;568:509-35. doi: 10.1016/bs.mie.2015.09.019. Epub 2015 Nov 3.
Current research utilizes the specific expression pattern of intermediate filaments (IF) for identifying cellular state and origin, as well as for the purpose of disease diagnosis. Nestin is commonly utilized as a specific marker and driver for CNS progenitor cell types, but in addition, nestin can be found in several mesenchymal progenitor cells, and it is constitutively expressed in a few restricted locations, such as muscle neuromuscular junctions and kidney podocytes. Alike most other members of the IF protein family, nestin filaments are dynamic, constantly being remodeled through posttranslational modifications, which alter the solubility, protein levels, and signaling capacity of the nestin filaments. Through its interactions with kinases and other signaling executors, resulting in a complex and bidirectional regulation of cell signaling events, nestin has the potential to determine whether cells divide, differentiate, migrate, or stay in place. In this review, the broad and similar roles of IFs as dynamic signaling scaffolds, is exemplified by observations of nestin functions and its interaction with the cyclin- dependent kinase 5, the atypical kinase in the family of cyclin-dependent kinases.
当前的研究利用中间丝（IF）的特定表达模式来识别细胞状态和起源，以及用于疾病诊断。巢蛋白通常用作中枢神经系统祖细胞类型的特异性标志物和驱动因子，但此外，巢蛋白也可在几种间充质祖细胞中发现，并且它在一些特定位置组成性表达，如肌肉神经肌肉接头和肾足细胞。与IF蛋白家族的大多数其他成员一样，巢蛋白丝是动态的，通过翻译后修饰不断重塑，这会改变巢蛋白丝的溶解度、蛋白质水平和信号传导能力。通过与激酶和其他信号传导执行者相互作用，从而对细胞信号事件进行复杂的双向调节，巢蛋白有可能决定细胞是分裂、分化、迁移还是保持原位。在本综述中，中间丝作为动态信号支架的广泛且相似的作用，通过巢蛋白功能及其与细胞周期蛋白依赖性激酶5（细胞周期蛋白依赖性激酶家族中的非典型激酶）相互作用的观察结果得以例证。
