Zuela Noam, Gruenbaum Yosef
Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Methods Enzymol. 2016;568:661-79. doi: 10.1016/bs.mie.2015.09.020. Epub 2015 Nov 3.
More than 70 different genes in humans and 12 different genes in Caenorhabditis elegans encode the superfamily of intermediate filament (IF) proteins. In C. elegans, similar to humans, these proteins are expressed in a cell- and tissue-specific manner, can assemble into heteropolymers and into 5-10nm wide filaments that account for the principal structural elements at the nuclear periphery, nucleoplasm, and cytoplasm. At least 5 of the 11 cytoplasmic IFs, as well as the nuclear IF, lamin, are essential. In this chapter, we will include a short review of our current knowledge of both cytoplasmic and nuclear IFs in C. elegans and will describe techniques used for their analyses.
人类中有70多种不同的基因以及秀丽隐杆线虫中有12种不同的基因编码中间丝（IF）蛋白超家族。在秀丽隐杆线虫中，与人类相似，这些蛋白以细胞和组织特异性的方式表达，能够组装成异源聚合物以及5 - 10纳米宽的细丝，这些细丝构成了核周、核质和细胞质中的主要结构成分。11种细胞质中间丝中的至少5种以及核中间丝核纤层蛋白是必不可少的。在本章中，我们将简要回顾一下目前我们对秀丽隐杆线虫细胞质和核中间丝的了解，并描述用于分析它们的技术。
