Buechner Matthew, Yang Zhe, Al-Hashimi Hikmat
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.
Deciphera, Pharmaceuticals, Inc., Lawrence, KS 66044, USA.
J Dev Biol. 2020 Sep 7;8(3):17. doi: 10.3390/jdb8030017.
Formation and regulation of properly sized epithelial tubes is essential for multicellular life. The excretory canal cell of provides a powerful model for investigating the integration of the cytoskeleton, intracellular transport, and organismal physiology to regulate the developmental processes of tube extension, lumen formation, and lumen diameter regulation in a narrow single cell. Multiple studies have provided new understanding of actin and intermediate filament cytoskeletal elements, vesicle transport, and the role of vacuolar ATPase in determining tube size. Most of the genes discovered have clear homologues in humans, with implications for understanding these processes in mammalian tissues such as Schwann cells, renal tubules, and brain vasculature. The results of several new genetic screens are described that provide a host of new targets for future studies in this informative structure.
形成并调控尺寸合适的上皮管对于多细胞生物的生存至关重要。秀丽隐杆线虫的排泄管细胞为研究细胞骨架、细胞内运输和机体生理学的整合提供了一个强大的模型，以调控狭窄单细胞中管延伸、管腔形成和管腔直径调节的发育过程。多项研究对肌动蛋白和中间丝细胞骨架成分、囊泡运输以及液泡ATP酶在确定管大小中的作用有了新的认识。发现的大多数基因在人类中有明确的同源物，这对于理解雪旺细胞、肾小管和脑血管等哺乳动物组织中的这些过程具有重要意义。本文描述了几个新的遗传筛选结果，为这个信息丰富的结构的未来研究提供了许多新的靶点。
