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甜玉米产量相关拥挤胁迫耐受性共表达网络的鉴定

Identification of Crowding Stress Tolerance Co-Expression Networks Involved in Sweet Corn Yield.

作者信息

Choe Eunsoo, Drnevich Jenny, Williams Martin M

机构信息

Global Change and Photosynthesis Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Urbana, Illinois, United States of America.

Roy J. Carver Biotechnology Center, University of Illinois, Urbana, Illinois, United States of America.

出版信息

PLoS One. 2016 Jan 21;11(1):e0147418. doi: 10.1371/journal.pone.0147418. eCollection 2016.

Abstract

Tolerance to crowding stress has played a crucial role in improving agronomic productivity in field corn; however, commercial sweet corn hybrids vary greatly in crowding stress tolerance. The objectives were to 1) explore transcriptional changes among sweet corn hybrids with differential yield under crowding stress, 2) identify relationships between phenotypic responses and gene expression patterns, and 3) identify groups of genes associated with yield and crowding stress tolerance. Under conditions of crowding stress, three high-yielding and three low-yielding sweet corn hybrids were grouped for transcriptional and phenotypic analyses. Transcriptional analyses identified from 372 to 859 common differentially expressed genes (DEGs) for each hybrid. Large gene expression pattern variation among hybrids and only 26 common DEGs across all hybrid comparisons were identified, suggesting each hybrid has a unique response to crowding stress. Over-represented biological functions of DEGs also differed among hybrids. Strong correlation was observed between: 1) modules with up-regulation in high-yielding hybrids and yield traits, and 2) modules with up-regulation in low-yielding hybrids and plant/ear traits. Modules linked with yield traits may be important crowding stress response mechanisms influencing crop yield. Functional analysis of the modules and common DEGs identified candidate crowding stress tolerant processes in photosynthesis, glycolysis, cell wall, carbohydrate/nitrogen metabolic process, chromatin, and transcription regulation. Moreover, these biological functions were greatly inter-connected, indicating the importance of improving the mechanisms as a network.

摘要

对拥挤胁迫的耐受性在提高大田玉米农艺生产力方面发挥了关键作用;然而,商业甜玉米杂交种在拥挤胁迫耐受性方面差异很大。本研究的目的是:1)探究在拥挤胁迫下产量不同的甜玉米杂交种之间的转录变化;2)确定表型反应与基因表达模式之间的关系;3)确定与产量和拥挤胁迫耐受性相关的基因组。在拥挤胁迫条件下,将三个高产和三个低产甜玉米杂交种分组进行转录和表型分析。转录分析确定每个杂交种有372至859个常见的差异表达基因(DEG)。杂交种之间存在较大的基因表达模式差异,并且在所有杂交种比较中仅鉴定出26个常见的DEG,这表明每个杂交种对拥挤胁迫都有独特的反应。杂交种之间DEG的过度表达生物学功能也有所不同。在以下两者之间观察到强相关性:1)高产杂交种中上调的模块与产量性状;2)低产杂交种中上调的模块与植株/果穗性状。与产量性状相关的模块可能是影响作物产量的重要拥挤胁迫反应机制。对这些模块和常见DEG的功能分析确定了光合作用、糖酵解、细胞壁、碳水化合物/氮代谢过程、染色质和转录调控等候选拥挤胁迫耐受过程。此外,这些生物学功能之间存在很大的相互联系,表明将这些机制作为一个网络进行改进的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f9f/4721684/1ef8bc7bd2d5/pone.0147418.g001.jpg

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