Tompkins L S
Curr Clin Top Infect Dis. 1989;10:174-93.
Few of the probes described here are available as practical assays for clinical laboratories, and some have argued that currently available kits have not lived up to their promise to provide increased sensitivity, specificity, and speed of identification and detection at a reasonable cost. While these sentiments are true to some extent now, the rapid advance of techniques to enhance the rate of hybridization, to amplify the target sequences, and to label and detect hybrids nonradioactively will have a substantial impact on the availability of hybridization kits for the clinical laboratory. It is conceivable that probe technology will be simplified to the extent that it can be adapted to small laboratories, clinics, and field sites. Not only can we expect probes to speed detection and identification of established pathogens, we also look forward to perhaps the most exciting future aspect of this technology, the discovery of new agents and new infectious disease syndromes that will be brought about through its application.
这里所描述的探针很少能作为临床实验室的实用检测方法，而且一些人认为，目前可用的试剂盒并未达到其承诺，即在合理成本下提供更高的灵敏度、特异性以及鉴定和检测速度。虽然目前在某种程度上这些观点是正确的，但提高杂交速率、扩增靶序列以及非放射性标记和检测杂交体的技术的迅速发展，将对临床实验室杂交试剂盒的可用性产生重大影响。可以想象，探针技术将被简化到能够适用于小型实验室、诊所和现场检测点的程度。我们不仅期望探针能加快对已确定病原体的检测和鉴定，我们还期待这项技术最令人兴奋的未来方面，即通过其应用发现新的病原体和新的传染病综合征。
