Gilbert G L, James G S, Sintchenko V
Centre for Infectious Diseases, Westmead Hospital, Sydney.
Med J Aust. 1999 Nov 15;171(10):536-9.
Nucleic acid detection methods, such as polymerase chain reaction (PCR), can often detect specific microbial pathogens, virulence markers and antimicrobial resistance genes more rapidly and with greater sensitivity and specificity than culture and conventional identification and susceptibility testing. Multiplex PCR can detect multiple genes in a single assay; this capability will be greatly extended by new techniques such as the DNA chip. However, limitations and pitfalls of nucleic detection methods remain.
核酸检测方法，如聚合酶链反应（PCR），通常比培养以及传统的鉴定和药敏试验能更快速地检测特定微生物病原体、毒力标志物和抗菌药物耐药基因，且具有更高的灵敏度和特异性。多重PCR可以在一次检测中检测多个基因；DNA芯片等新技术将极大地扩展这一能力。然而，核酸检测方法仍存在局限性和缺陷。
