Bi Yalan, Meng Yunxiao, Wu Huanwen, Cui Quancai, Luo Yufeng, Xue Xiaowei
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Surg Oncol. 2016 Feb;113(2):144-51. doi: 10.1002/jso.24124. Epub 2016 Jan 12.
To investigate the expression profiles of cancer stem cells (CSCs) markers CD133 and CD44 in a cohort of medullary thyroid carcinoma (MTC) patients, and their prognostic values during 10-year follow-up.
MTC samples were obtained for H&E and immunohistochemical analysis. Survival analysis was performed using Kaplan-Meier method and log-rank test.
Both the CD133 and CD44 positives were higher in MTC than control. High expression of CD133 and CD44 was positively correlated with capsule invasion and each other, and their co-expression was significantly correlated with capsule invasion, tissue invasion, and metastases at surgery. Tumor size, capsular invasion, tissue invasion, metastases at surgery, surgical plan, lymph node metastases, TNM stage, CD133, and CD44 were prognostic factors for overall survival (OS) and/or disease free survival (DFS). Both the CD133 and CD44 were unfavorable prognostic predictors for OS (P = 0.046, P = 0.03), while only CD44 was a significant predictor for DFS (P = 0.017). OS rate in CD133/CD44 co-expression group was significantly lower than that in non-co-expression group (χ(2) = 8.44, P = 0.004).
Our study suggested the high expression of CD133 and CD44 in the MTC, and CD133 and CD44 expressions were correlated with capsule invasion and with OS. CD133 and/or CD44 may be prognostic factors for OS and/or DFS in our MTC patients.
研究髓样甲状腺癌（MTC）患者队列中癌症干细胞（CSCs）标志物CD133和CD44的表达谱及其在10年随访期间的预后价值。
获取MTC样本进行苏木精-伊红（H&E）和免疫组织化学分析。采用Kaplan-Meier法和对数秩检验进行生存分析。
MTC中CD133和CD44阳性率均高于对照组。CD133和CD44的高表达与包膜侵犯呈正相关且两者之间也呈正相关，它们的共表达与手术时的包膜侵犯、组织侵犯和转移显著相关。肿瘤大小、包膜侵犯、组织侵犯、手术时转移、手术方案、淋巴结转移、TNM分期、CD133和CD44是总生存期（OS）和/或无病生存期（DFS）的预后因素。CD133和CD44均是OS的不良预后预测指标（P = 0.046，P = 0.03），而只有CD44是DFS的显著预测指标（P = 0.017）。CD133/CD44共表达组的OS率显著低于非共表达组（χ(2) = 8.44，P = 0.004）。
我们的研究表明MTC中CD133和CD44高表达，且CD133和CD44表达与包膜侵犯及OS相关。CD133和/或CD44可能是我们研究的MTC患者OS和/或DFS的预后因素。
