Procházka R, Motlík J, Fulka J
Czechoslovak Academy of Sciences, Institute of Animal Physiology and Genetics, Department of Genetics, Libĕchov.
Cell Differ Dev. 1989 Sep;27(3):175-81. doi: 10.1016/0922-3371(89)90698-9.
Microinjection of 120 pl homologous or heterologous (mouse) maturing cytoplasm into immature pig oocytes resulted in germinal vesicle breakdown (GVBD) and chromosome condensation (CC) after 8 h of culture. In contrast, nearly all control oocytes remained at the germinal vesicle stage. Immature cytoplasm did not induce nuclear maturation of recipient oocytes when injected. The cytoplasm of the oocytes injected with maturing cytoplasm preserved the capacity to cause GVBD in the second recipients. These results suggest that the level of injected maturation promoting factor (MPF) must rise before GVBD can occur. When the pig oocytes injected with 120 pl of mouse maturing cytoplasm were cultured in cycloheximide for 8 h, the presence of GVBD was dramatically reduced. Thus, in pig oocytes the increase in active MPF up to the effective concentration requires protein synthesis.
将120微升同源或异源（小鼠）成熟细胞质显微注射到未成熟猪卵母细胞中，培养8小时后导致生发泡破裂（GVBD）和染色体凝聚（CC）。相比之下，几乎所有对照卵母细胞都停留在生发泡阶段。未成熟细胞质注射时不会诱导受体卵母细胞核成熟。注射了成熟细胞质的卵母细胞的细胞质保留了在第二个受体中引起GVBD的能力。这些结果表明，在GVBD发生之前，注射的成熟促进因子（MPF）水平必须升高。当注射了120微升小鼠成熟细胞质的猪卵母细胞在放线菌酮中培养8小时时，GVBD的出现显著减少。因此，在猪卵母细胞中，活性MPF增加到有效浓度需要蛋白质合成。
