[从老挝人民民主共和国腹泻儿童中分离出的产肠毒素大肠杆菌菌株的特性分析]
[Characterization of the enterotoxigenic strains of Escherichia coli isolated from children with diarrhea in Popular Democratic Republic Lao].
作者信息
Germani Y, Insisiengmay S, Boupha P, Mixap B, Khouan T, Le Gonidec G
机构信息
Institut Pasteur de Nouméa, Laboratoire des Bactéries Entéropathogènes, Nouvelle-Calédonie.
出版信息
Bull Soc Pathol Exot Filiales. 1989;82(4):465-75.
Enterotoxigenic Escherichia coli (ETEC) of infant origin from the Popular Democratic Republic Lao were characterized with respect to there 0 serogroups, biotypes, anti-bioresistances, fimbrial antigens and types of enterotoxins produced. Enterotoxin production was determined by the suckling mice assay, competitive GM1-erythroassay, and cell cultures (CHOK1 and Y1). The presence of genes encoding for the enterotoxins was determined by colony hybridization by using radioactive DNA probes. Profile plasmids from ETEC strains were studied. The plasmids encoding for heat-labile enterotoxin were studied with an acetyl-aminofluorene modified probe.
对来自老挝人民民主共和国的婴儿源产肠毒素大肠杆菌（ETEC）进行了0血清群、生物型、抗药性、菌毛抗原和所产肠毒素类型方面的特征分析。通过乳鼠试验、竞争性GM1-红细胞试验和细胞培养（CHOK1和Y1）来测定肠毒素的产生。利用放射性DNA探针通过菌落杂交来测定编码肠毒素的基因的存在情况。对ETEC菌株的质粒图谱进行了研究。用乙酰氨基芴修饰的探针研究了编码不耐热肠毒素的质粒。
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