一种使用寡核苷酸和多核苷酸探针鉴定产肠毒素大肠杆菌的非放射性化学发光方法的评估。
Evaluation of a non-radioactive chemiluminescent method for using oligonucleotide and polynucleotide probes to identify enterotoxigenic Escherichia coli.
作者信息
Abdul Alim A, Faruque S M, Ahmad Q S, Hossain K M, Mahalanabis D, Albert M J
机构信息
Molecular Biology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
出版信息
J Diarrhoeal Dis Res. 1994 Jun;12(2):113-6.
We compared the applicability of an enhanced chemiluminescent (ECL) method for using gene probes with that of radioactive probes to identify enterotoxigenic Escherichia coli (ETEC) in stools of Bangladeshi children with diarrhoea. Colony blots of E. coli isolates were hybridized using both [alpha-32P]-dCTP labelled and fluorescein-11-dUTP labelled polynucleotide and oligonucleotide gene probes for heat-labile enterotoxin (LT), and heat-stable enterotoxin (ST). Analysis of 1,620 isolates obtained from 540 patients gave similar results by both radioactive and chemiluminescent probes. The ECL method was faster than the radioactive method. Both polynucleotide and oligonucleotide probes could be used by the ECL method. Hybridization and detection by the ECL method appeared to be a convenient alternative to radioactive probes for screening E. coli isolates for ETEC.
我们比较了增强化学发光（ECL）方法使用基因探针与放射性探针在鉴定孟加拉国腹泻儿童粪便中产肠毒素大肠杆菌（ETEC）方面的适用性。使用[α-32P]-dCTP标记和荧光素-11-dUTP标记的多核苷酸及寡核苷酸基因探针，对大肠杆菌分离株的菌落印迹进行热不稳定肠毒素（LT）和热稳定肠毒素（ST）的杂交。对从540名患者获得的1620株分离株进行分析，放射性探针和化学发光探针得到的结果相似。ECL方法比放射性方法更快。ECL方法可以使用多核苷酸和寡核苷酸探针。对于筛选大肠杆菌分离株中的ETEC，ECL方法的杂交和检测似乎是放射性探针的一种方便替代方法。
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