Sun Mingzhe, Jia Bowei, Cui Na, Wen Yidong, Duanmu Huizi, Yu Qingyue, Xiao Jialei, Sun Xiaoli, Zhu Yanming
Key Laboratory of Agricultural Biological Functional Gene, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, 163319, People's Republic of China.
Plant Mol Biol. 2016 Mar;90(4-5):419-34. doi: 10.1007/s11103-015-0426-7. Epub 2016 Jan 22.
It is widely accepted that Ca(2+)ATPase family proteins play important roles in plant environmental stress responses. However, up to now, most researches are limited in the reference plants Arabidopsis and rice. The function of Ca(2+)ATPases from non-reference plants was rarely reported, especially its regulatory role in carbonate alkaline stress responses. Hence, in this study, we identified the P-type II Ca(2+)ATPase family genes in soybean genome, determined their chromosomal location and gene architecture, and analyzed their amino acid sequence and evolutionary relationship. Based on above results, we pointed out the existence of gene duplication for soybean Ca(2+)ATPases. Then, we investigated the expression profiles of the ACA subfamily genes in wild soybean (Glycine soja) under carbonate alkaline stress, and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses, as evidenced by lower levels of membrane permeability and MDA content, but higher levels of SOD activity, proline concentration and chlorophyll content under stress conditions. Taken together, for the first time, we reported a P-type II Ca(2+)ATPase from wild soybean, GsACA1, which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.
人们普遍认为，Ca(2+)ATPase家族蛋白在植物环境胁迫响应中发挥重要作用。然而，截至目前，大多数研究局限于模式植物拟南芥和水稻。非模式植物中Ca(2+)ATPases的功能鲜有报道，尤其是其在碳酸盐碱性胁迫响应中的调控作用。因此，在本研究中，我们鉴定了大豆基因组中的P型II Ca(2+)ATPase家族基因，确定了它们的染色体定位和基因结构，并分析了它们的氨基酸序列和进化关系。基于上述结果，我们指出大豆Ca(2+)ATPases存在基因复制现象。然后，我们研究了野生大豆（Glycine soja）中ACA亚家族基因在碳酸盐碱性胁迫下的表达谱，并通过转基因苜蓿对一个代表性基因GsACA1进行了功能表征。我们的结果表明，在苜蓿中过表达GsACA1显著提高了植物对碳酸盐碱性胁迫和中性盐胁迫的耐受性，胁迫条件下膜透性和丙二醛含量较低、超氧化物歧化酶活性、脯氨酸浓度和叶绿素含量较高证明了这一点。综上所述，我们首次报道了来自野生大豆的一种P型II Ca(2+)ATPase，即GsACA1，它可以正向调控植物对碳酸盐碱性胁迫和中性盐胁迫的耐受性。
