Genome-Wide Identification and Expression Analysis of /s in L.

Pepper ( L.) is a popular vegetable in people's daily lives. During pepper growth, calcium (Ca) is an essential macronutrient, and calcium-transporting ATPase (ACA/ECA) is a vital protein for calcium transport. However, reports on the / gene family in the pepper genome are lacking. Hence, we used various bioinformatics methods to identify the / gene family in pepper. We identified eleven /-family genes in pepper. The chromosomal distribution, phylogenetic evolution, characteristics, gene collinearity, gene and protein structures, cis-acting elements, and specific expression patterns of /s were analyzed, revealing evolutionary relationships and correlations between /s and other species (Arabidopsis, rice, and tomato). The experimental results indicate that are stable and hydrophobic proteins, with each of the eleven proteins containing all ten motifs. Eleven / genes are unevenly distributed on the eight chromosomes, and they substantially differ in the number of exons. We found a close correlation between the /s of pepper, Arabidopsis, and tomato. The / genes contain various plant-hormone-, growth-, and stress-related cis-acting elements. The qRT-PCR results indicate that the expression levels of the eleven /s exhibit differential temporal expression patterns under various exogenous Ca concentrations. These results provide a theoretical basis for further studying the function of the pepper / gene family and valuable information for identifying and screening genes for pepper stress tolerance breeding.