Li M, Li Y J
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1989;7(2):85-8.
Circulating immune complexes (CIC) isolated from sera of mice infected with Plasmodium berghei by precipitation with polyethylene glycol were examined for their ability to stimulate the production of reactive oxygen species by peritoneal exudate (PEC) of normal mice, using luminol-aided chemiluminescence (CL). CIC were found to be capable of stimulating the production of CL by normal mouse PEC. Weaker CL responses were observed when PEC were incubated with P. berghei soluble antigens. Normal mouse IgG and IgG1, IgG2b subclasses showed no effect on the stimulation of the production of CL. When normal mouse PEC were preincubated with CIC, their CL responses to opsonized zymosan were significantly depressed to 34% of the control level. The data suggest that CIC are capable of stimulating phagocytes to release reactive oxygen species and mediating pathological tissue damage.
通过聚乙二醇沉淀从感染伯氏疟原虫的小鼠血清中分离出循环免疫复合物（CIC），利用鲁米诺辅助化学发光（CL）检测其刺激正常小鼠腹腔渗出液（PEC）产生活性氧的能力。发现CIC能够刺激正常小鼠PEC产生CL。当PEC与伯氏疟原虫可溶性抗原孵育时，观察到较弱的CL反应。正常小鼠IgG及IgG1、IgG2b亚类对CL产生的刺激无影响。当正常小鼠PEC与CIC预孵育时，它们对调理酵母聚糖的CL反应显著降低至对照水平的34%。数据表明，CIC能够刺激吞噬细胞释放活性氧并介导病理性组织损伤。
