Descamps-Latscha B, Nguyen A T, Mouton D
INSERM U 25, Hôpital Necker, Paris, France.
Exp Clin Immunogenet. 1988;5(4):226-37.
The present study is an attempt to verify the hypothesis that high (HI) and low (LI) antibody responder Biozzi mice, which differ strikingly in their macrophage capacities to catabolize antigens and to exert bactericidal activity (the LI being more potent than the HI line), demonstrate a similar genetic disparity in their macrophage oxidative metabolism activation potential. The production of reactive oxygen intermediates was measured by luminol-dependent chemiluminescence in cell suspensions from various sources including: bone marrow, peripheral blood, spleen, peritoneal exudate, thymus, and mesenteric lymph nodes. Chemiluminescence was determined both in the absence and presence of particulate (opsonized zymosan and latex) and soluble (phorbol myristate acetate and concanavalin A) membrane-stimulating agents. Lastly this investigation was conducted both in normal and endotoxin (lipopolysaccharide from Escherichia coli) treated HI and LI mice in an attempt to unmask the hypothesized difference by increasing the macrophage oxidative response capacity. Results obtained did not show the expected interline differences in reactive oxygen intermediate production regardless of cell origin, stimulating agent or lipopolysaccharide in vivo treatment. On the other hand, the well-documented enhancing effect of lipopolysaccharide on macrophage oxidative metabolism depended on the origin of the cell suspension. Indeed, it was not observed in bone marrow and peripheral blood from either HI or LI mice, was clearly demonstrated in the spleen and peritoneal exudate from both mouse lines, and surprisingly, in the thymus and lymph nodes from HI but not LI mice.
高抗体应答（HI）和低抗体应答（LI）的Biozzi小鼠在巨噬细胞分解代谢抗原及发挥杀菌活性的能力上存在显著差异（LI系比HI系更具活性），它们在巨噬细胞氧化代谢激活潜能方面也表现出类似的遗传差异。通过鲁米诺依赖的化学发光法测定来自各种来源的细胞悬液中产生活性氧中间体的情况，这些来源包括：骨髓、外周血、脾脏、腹腔渗出液、胸腺和肠系膜淋巴结。在不存在和存在颗粒性（调理酵母聚糖和乳胶）及可溶性（佛波酯和伴刀豆球蛋白A）膜刺激剂的情况下测定化学发光。最后，在正常及经内毒素（大肠杆菌来源的脂多糖）处理的HI和LI小鼠中进行此项研究，试图通过增强巨噬细胞氧化反应能力来揭示假设的差异。无论细胞来源、刺激剂或体内脂多糖处理情况如何，所获得的结果均未显示活性氧中间体产生方面预期的品系间差异。另一方面，脂多糖对巨噬细胞氧化代谢的增强作用取决于细胞悬液的来源。实际上，在HI或LI小鼠的骨髓和外周血中未观察到这种作用，在两个品系小鼠的脾脏和腹腔渗出液中均明显表现出这种作用，而且令人惊讶的是，在HI小鼠而非LI小鼠的胸腺和淋巴结中也观察到这种作用。
