Cintra M C R, Teixeira V N, Nascimento L V, Sotomaior C S
Graduate Program in Animal Science, School of Agricultural Sciences and Veterinary Medicine-Pontifícia Universidade Católica do Paraná (PUCPR), São José dos Pinhais, Paraná 83010-500, Brazil.
School of Agricultural Sciences and Veterinary Medicine-Pontifícia Universidade Católica do Paraná (PUCPR), Brazil.
Vet Parasitol. 2016 Jan 30;216:4-6. doi: 10.1016/j.vetpar.2015.11.013. Epub 2015 Nov 28.
Multiple drug resistance of nematodes against anthelmintics has become one of the most important economic problems in sheep production worldwide. The aim of this experiment was to evaluate the efficacy of monepantel (2.5mg/kg) against gastrointestinal nematodes in fecal egg count reduction test (FECRT) and controlled efficacy test (CT) in naturally infected sheep. We used 30 sheep for the FECRT and 20 sheep for the CT, equally divided into control and treated groups. In the FECRT, the reduction was 98%. Larval identification of pre-treatment coprocultures revealed 100% Haemonchus spp. for both control and treated groups. Post-treatment culture of treated sheep was 100% Oesophagostomum spp., but only few larvae were recovered. In the control group, they were 99% Haemonchus spp and 1% Oesophagostomum spp. larvae. Based on the FECRT, Haemonchus spp. was considered susceptible to monepantel. The efficacy of monepantel in the CT against Haemonchus contortus and Trichostrongylus axei was 100% and against Cooperia curticei was 99.7%. For Trichostrongylus colubriformis, the efficacy was -21.5%. In both treated and untreated animals, Oesophagostomum columbianum was recovered from the large intestines. Based on FECRT and CT and in accordance with WAAVP standards, monepantel was ineffective against T. colubriformis and O. columbianum, but effective against H. contortus, T. axei and C. curticei in the studied flock.
线虫对驱虫药的多重耐药性已成为全球绵羊生产中最重要的经济问题之一。本实验的目的是在自然感染的绵羊中，通过粪便虫卵计数减少试验（FECRT）和对照药效试验（CT）评估莫能菌素（2.5mg/kg）对胃肠道线虫的疗效。我们在FECRT中使用了30只绵羊，在CT中使用了20只绵羊，均分为对照组和治疗组。在FECRT中，虫卵减少率为98%。预处理粪便培养物的幼虫鉴定显示，对照组和治疗组的捻转血矛线虫均为100%。治疗后绵羊的培养物中100%为哥伦比亚食道口线虫，但仅回收了少数幼虫。对照组中，捻转血矛线虫幼虫为99%，哥伦比亚食道口线虫幼虫为1%。基于FECRT，捻转血矛线虫被认为对莫能菌素敏感。莫能菌素在CT中对捻转血矛线虫和奥氏奥斯特线虫的疗效为100%，对柯氏古柏线虫的疗效为99.7%。对蛇形毛圆线虫的疗效为-21.5%。在治疗和未治疗的动物中，均从大肠中回收了哥伦比亚食道口线虫。根据FECRT和CT以及WAAVP标准，莫能菌素对蛇形毛圆线虫和哥伦比亚食道口线虫无效，但对研究羊群中的捻转血矛线虫、奥氏奥斯特线虫和柯氏古柏线虫有效。
