Malatji D P, Tsotetsi A M, van Marle-Koster E, Muchadeyi F C
Biotechnology Platform, Agricultural Research Council, Onderstepoort, South Africa; Department of Wildlife and Animal Science, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria, South Africa.
Parasites, Vectors and Vector-borne Diseases Program, Agricultural Research Council, Onderstepoort, South Africa; Department of Zoology and Entomology, Faculty of Natural Sciences, University of Free State (Qwa-qwa campus), South Africa.
Vet Parasitol. 2016 Jan 30;216:89-92. doi: 10.1016/j.vetpar.2015.12.012. Epub 2015 Dec 18.
Ascaridia galli is one of the most common nematode affecting chickens. This study characterized A. galli parasites collected from South African village chickens of Limpopo (n=18) and KwaZulu-Natal (n=22) provinces using the 510bp sequences of cytochrome C oxidase subunit 1 gene of the mitochondrial DNA. Fourteen and 12 polymorphic sites were observed for Limpopo and KwaZulu-Natal sequences, respectively. Six haplotypes were observed in total. Haplotype diversity was high and ranged from 0.749 for Limpopo province to 0.758 for KwaZulu-Natal province isolates. There was no genetic differentiation between A. galli from Limpopo and KwaZulu-Natal provinces. The six South African haplotypes were unique compared to those published in the GeneBank sampled from Hy-line chickens raised under organic farming in Denmark. The utility of cytochrome C oxidase subunit 1 gene as a potential genetic marker for studying A. galli in village chicken populations is presented.
鸡蛔虫是感染鸡的最常见线虫之一。本研究利用线粒体DNA细胞色素C氧化酶亚基1基因的510bp序列，对从林波波省（n = 18）和夸祖鲁 - 纳塔尔省（n = 22）的南非乡村鸡中收集的鸡蛔虫寄生虫进行了特征分析。林波波省和夸祖鲁 - 纳塔尔省序列分别观察到14个和12个多态性位点。总共观察到6种单倍型。单倍型多样性较高，林波波省分离株为0.749，夸祖鲁 - 纳塔尔省分离株为0.758。林波波省和夸祖鲁 - 纳塔尔省的鸡蛔虫之间没有遗传分化。与丹麦有机养殖的海兰鸡在基因库中公布的单倍型相比，这六种南非单倍型是独特的。本文介绍了细胞色素C氧化酶亚基1基因作为研究乡村鸡群中鸡蛔虫潜在遗传标记的实用性。
