Urbanowicz Joanna, Gaweł Andrzej, Bobrek Kamila
Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wrocław, Poland.
Acta Parasitol. 2018 Sep 25;63(3):640-644. doi: 10.1515/ap-2018-0073.
Ascaridia (A.) galli is one of the most commonly occurring nematodes in poultry worldwide, often in hens and broiler chickens. The infection with Ascaridia galli in free-range chickens was even 70%. There is not much information about A. galli genetic features. The present study was conducted to assess the genetic diversity of A. galli isolated from hens in Poland by analyzing the nucleotide sequence of the region ITS1-5.8rRNA-ITS2 and to define its homology within the family Ascaridiidae. Adult A. galli were collected from the intestines of naturally infected hens from two flocks of free-run laying hens from the Wielkopolska region in Poland. From all parasites an identical ITS1-5.8rRNA-ITS2 sequence was obtained, which was homologous in 99% with A. columbae (JQ995321.1) sequence. The high homology sequences of A. galli (KX683286) from Poland and A. columbae (JQ995321.1) isolate from the USA, support the observations of other authors suggesting that A. galli and A. columbae might be closely related. It is the first whole ITS1-5.8rRNA-ITS2 of A. galli in the GenBank database, so there is not enough data for detailed phylogenetic analysis of A. galli. Detailed genetic analysis is necessary to get better insight into the birds' Ascaridia species.
鸡蛔虫是全球家禽中最常见的线虫之一，常见于母鸡和肉鸡体内。散养鸡的鸡蛔虫感染率甚至高达70%。关于鸡蛔虫的遗传特征，目前所知信息不多。本研究通过分析ITS1-5.8rRNA-ITS2区域的核苷酸序列，对从波兰母鸡体内分离出的鸡蛔虫进行遗传多样性评估，并确定其在鸡蛔虫科内的同源性。成年鸡蛔虫是从波兰大波兰地区两群自由放养蛋鸡的自然感染母鸡肠道中采集的。从所有寄生虫中获得了相同的ITS1-5.8rRNA-ITS2序列，该序列与鸽蛔虫（JQ995321.1）序列的同源性为99%。来自波兰的鸡蛔虫（KX683286）与来自美国的鸽蛔虫（JQ995321.1）的高同源序列，支持了其他作者的观察结果，即鸡蛔虫和鸽蛔虫可能密切相关。这是GenBank数据库中鸡蛔虫的首个完整ITS1-5.8rRNA-ITS2序列，因此没有足够的数据对鸡蛔虫进行详细的系统发育分析。为了更深入了解鸟类的蛔虫种类，进行详细的遗传分析是必要的。
