Ogawa M M, Hashimoto T, Nishikawa T, Castro R M
Clin Exp Dermatol. 1989 Jan;14(1):29-31. doi: 10.1111/j.1365-2230.1989.tb00878.x.
Human IgG possesses four main subclasses, namely IgG1, IgG2, IgG3, and IgG4, of these IgG1-IgG3 fix complement, but IgG4 does not. We have studied the IgG subclasses of intercellular antibodies in the sera from 20 patients with Brazilian pemphigus foliaceus by immunofluorescent staining using mouse monoclonal antibodies against human IgG1-IgG4. At the same time, the complement fixing capability of each antibody was examined by complement immunofluorescence. All of four subclasses were frequently detected in most cases with varying distributions. However, no specific pattern was observed. Complement fixing antibodies were found in four patients. However, the distribution of IgG subclasses was incompatible with their known characteristics in terms of complement activation. This discrepancy increases the controversy over the importance of the complement system in blister formation in pemphigus.
人免疫球蛋白G（IgG）有四个主要亚类，即IgG1、IgG2、IgG3和IgG4，其中IgG1 - IgG3可固定补体，但IgG4不能。我们用抗人IgG1 - IgG4的小鼠单克隆抗体通过免疫荧光染色研究了20例巴西落叶型天疱疮患者血清中细胞间抗体的IgG亚类。同时，通过补体免疫荧光检测每种抗体的补体固定能力。在大多数病例中，所有四个亚类均经常被检测到，分布各异。然而，未观察到特定模式。在四名患者中发现了补体固定抗体。然而，就补体激活而言，IgG亚类的分布与其已知特征不相符。这种差异增加了关于补体系统在天疱疮水疱形成中的重要性的争议。
